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Measurement of HNE-protein adducts in plasma and serum by ELISA


Weber, Daniela; Milkovic, Lidija; Zarkovic, Neven; Breusing, Nicolle; Grune, Tilman.
Measurement of HNE-protein adducts in plasma and serum by ELISA // SYMPOSIUM "BIOMARKERS OF AGEING" 2013
Konstanz, Njemačka, 2013. (poster, nije recenziran, sažetak, znanstveni)


Naslov
Measurement of HNE-protein adducts in plasma and serum by ELISA

Autori
Weber, Daniela ; Milkovic, Lidija ; Zarkovic, Neven ; Breusing, Nicolle ; Grune, Tilman.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
SYMPOSIUM "BIOMARKERS OF AGEING" 2013 / - , 2013

Skup
SYMPOSIUM "BIOMARKERS OF AGEING" 2013

Mjesto i datum
Konstanz, Njemačka, 22.-23.03.2013

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
HNE-protein adducts; ELISA; plasma; serum

Sažetak
The HNE-protein adduct ELISA is a method to detect HNE bound to proteins, which is considered as the most likely form of HNE occurrence in living systems. The aim of this work was to adapt the ELISA to a commercial antibody, as well as for non-commercial antibody, and to apply it in the analysis of human plasma/serum samples. After modification and validation of the protocol with both antibodies, samples of two groups were analyzed: apparently healthy obese (n=62) and non-obese controls (n=15). Although the detected absolute values of HNE-protein adducts were different, depending on the antibody used, both ELISA methods showed significantly higher values of HNE-protein adducts in the obese group.

Izvorni jezik
Engleski



POVEZANOST RADA


Projekt / tema
098-0982464-2519 - Lipidi, slobodni radikali i njihovi glasnici u integrativnoj onkologiji (Neven Žarković, )

Ustanove
Institut "Ruđer Bošković", Zagreb