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Effect of nimodipine on EEG activity and EEG power spectra in kainic acid-induced seizures in rats

Kainic acid (KA) is an excitatory glutaminergic agonist that given intrahippocampally induces seizures. The EEG changes show major spike activity originating in limbic system. The aim of our study was to investigate whether nimodipine could prevent KA-induced changes in EEG activity. Experiments were performed on adult Wistar rats. They were anesthetized and placed in a stereotaxic apparatus. Vehicle or nimodipine (30mg/kg) had been given 30 minutes prior to intrahippocampal injection of KA (15 nmol). In each rat four screw electrodes were: placed over the frontal and parietal cortex. The EEG was recorded in basal conditions and 5, 10, 15, 30 and 60 minutes after KA administration. In order to obtain EEG samples for frequency analysis, the output from the EEG machine was amplified and was transmitted to all IBM AT-compatible microcomputer via analog-to- digital converter. The system was calibrated using 500 V. Two 7.5 s samples of artefact free EEG were analyzed. In order to obtain power spectra between 0-30 Hz spectral averages were computed using Fast Fourier Transform. Individual EEG power of each frequency bin was normalized to average total power of baseline EEG frequencies were collapsed into 1 Hz bins. Our results demonstrate that nimodipine was effective in the prevention of changes in EEG activity induced by intrahippocampal KA administration.

nimodipine; kainic acid; EEG; rat

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