Nalazite se na CroRIS probnoj okolini. Ovdje evidentirani podaci neće biti pohranjeni u Informacijskom sustavu znanosti RH. Ako je ovo greška, CroRIS produkcijskoj okolini moguće je pristupi putem poveznice www.croris.hr
izvor podataka: crosbi !

AICAR induces differentiation of acute myeloid leukemia cells (CROSBI ID 603802)

Prilog sa skupa u časopisu | sažetak izlaganja sa skupa | međunarodna recenzija

Lalić, Hrvoje ; Dembitz, Vilma ; Lukinović-Škudar, Vesna ; Banfić, Hrvoje ; Višnjić, Dora AICAR induces differentiation of acute myeloid leukemia cells // Periodicum biologorum / Vitale, Branko (ur.). 2013. str. 38-x

Podaci o odgovornosti

Lalić, Hrvoje ; Dembitz, Vilma ; Lukinović-Škudar, Vesna ; Banfić, Hrvoje ; Višnjić, Dora

engleski

AICAR induces differentiation of acute myeloid leukemia cells

Pharmacological modulators of AMP-activated protein kinase (AMPK)/mammalian target of rapamycin (mTOR) pathway have been reported to exert antiproliferative effects in leukemia, but their role in differentiation is less explored. The aim of this study was to test for the effects of AMPK/ mTOR modulators in combination with differentiating agents in AML, especially in non- APL cell lines. Rapamycin alone had cytostatic effects but enhanced ATRA-mediated differentiation and DMSO- mediated growth arrest and apoptosis. AMPK activators, AICAR and metformin, significantly reduce the number of viable cells. Morphologic and FACS analysis shows the significant increase in apoptosis in cells treated with AICAR. Western blot analysis confirms that both agents activate AMPK and inhibit mTOR activity. AICAR enhances ATRA-mediated differentiation of NB4, a typical APL cell line. In U937 cells, AICAR alone induces the expression of cell surface markers associated with mature monocytes and macrophages. In both cell lines, AICAR increases the activity of MAPK, and the increase in the expression of CD64 and CD14 is inhibited in the presence of MAPK and AMPK inhibitors. These results suggest that a strategy using combination of differentiation agents with drugs targeting AMPK/mTOR may improve differentiation therapy for non-APL AML.

AICAR; differentiation; acute myeloid leukemia

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

Podaci o prilogu

38-x.

2013.

nije evidentirano

objavljeno

Podaci o matičnoj publikaciji

Periodicum biologorum

Vitale, Branko

Zagreb:

0031-5362

Podaci o skupu

3. Congress of Croatian Physiological Society and 1. Regional Congress of the Physiological Societies

poster

13.09.2013-15.09.2013

Rijeka, Hrvatska

Povezanost rada

Temeljne medicinske znanosti

Indeksiranost