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AICAR induces differentiation of acute myeloid leukemia cells


Lalić, Hrvoje; Dembitz, Vilma; Lukinović-Škudar, Vesna; Banfić, Hrvoje; Višnjić, Dora
AICAR induces differentiation of acute myeloid leukemia cells // Periodicum biologorum, Vol 115, Suppl 2, 2013. - 3.Congress of Croatian Physiological Society and 1.Regional Congress of the Physiological Societies / Vitale, Branko (ur.).
Zagreb, 2013. (poster, međunarodna recenzija, sažetak, znanstveni)


Naslov
AICAR induces differentiation of acute myeloid leukemia cells

Autori
Lalić, Hrvoje ; Dembitz, Vilma ; Lukinović-Škudar, Vesna ; Banfić, Hrvoje ; Višnjić, Dora

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Periodicum biologorum, Vol 115, Suppl 2, 2013. - 3.Congress of Croatian Physiological Society and 1.Regional Congress of the Physiological Societies / Vitale, Branko - Zagreb, 2013

Skup
3.Congress of Croatian Physiological Society and 1.Regional Congress of the Physiological Societies

Mjesto i datum
Rijeka, Hrvatska, 13-15.09.2013

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
AICAR; differentiation; acute myeloid leukemia

Sažetak
Pharmacological modulators of AMP-activated protein kinase (AMPK)/mammalian target of rapamycin (mTOR) pathway have been reported to exert antiproliferative effects in leukemia, but their role in differentiation is less explored. The aim of this study was to test for the effects of AMPK/ mTOR modulators in combination with differentiating agents in AML, especially in non- APL cell lines. Rapamycin alone had cytostatic effects but enhanced ATRA-mediated differentiation and DMSO- mediated growth arrest and apoptosis. AMPK activators, AICAR and metformin, significantly reduce the number of viable cells. Morphologic and FACS analysis shows the significant increase in apoptosis in cells treated with AICAR. Western blot analysis confirms that both agents activate AMPK and inhibit mTOR activity. AICAR enhances ATRA-mediated differentiation of NB4, a typical APL cell line. In U937 cells, AICAR alone induces the expression of cell surface markers associated with mature monocytes and macrophages. In both cell lines, AICAR increases the activity of MAPK, and the increase in the expression of CD64 and CD14 is inhibited in the presence of MAPK and AMPK inhibitors. These results suggest that a strategy using combination of differentiation agents with drugs targeting AMPK/mTOR may improve differentiation therapy for non-APL AML.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
108-1081347-0173 - Funkcija fosfoinozitol 3-kinaze C2 beta u staničnim jezgrama (Hrvoje Banfić, )
108-1081347-1448 - Uloga PLC i Akt u staničnom ciklusu i diferencijaciji leukemija (Dora Višnjić, )

Ustanove
Medicinski fakultet, Zagreb

Citiraj ovu publikaciju

Lalić, Hrvoje; Dembitz, Vilma; Lukinović-Škudar, Vesna; Banfić, Hrvoje; Višnjić, Dora
AICAR induces differentiation of acute myeloid leukemia cells // Periodicum biologorum, Vol 115, Suppl 2, 2013. - 3.Congress of Croatian Physiological Society and 1.Regional Congress of the Physiological Societies / Vitale, Branko (ur.).
Zagreb, 2013. (poster, međunarodna recenzija, sažetak, znanstveni)
Lalić, H., Dembitz, V., Lukinović-Škudar, V., Banfić, H. & Višnjić, D. (2013) AICAR induces differentiation of acute myeloid leukemia cells. U: Vitale, B. (ur.)Periodicum biologorum, Vol 115, Suppl 2, 2013. - 3.Congress of Croatian Physiological Society and 1.Regional Congress of the Physiological Societies.
@article{article, editor = {Vitale, B.}, year = {2013}, pages = {38}, keywords = {AICAR, differentiation, acute myeloid leukemia}, title = {AICAR induces differentiation of acute myeloid leukemia cells}, keyword = {AICAR, differentiation, acute myeloid leukemia}, publisherplace = {Rijeka, Hrvatska} }

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus