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Nucleophosmin cytoplasmic and nucleus immunohistochemical cell positivity of bone marrow biopsy sections in AML and MDS patients


Sučić, Mirna; Ilić, Ivana; Dotlić; Snježana; Labar, Boris; Ljubić, Nives; Županić Krmek, Dubravka
Nucleophosmin cytoplasmic and nucleus immunohistochemical cell positivity of bone marrow biopsy sections in AML and MDS patients // Leukemia Research, Suppl.37, 2013 / Bennett, John M ; Feldman, Eric J (ur.).
Oxford, UK: Elsevier, 2013. str. S135-s136 (poster, međunarodna recenzija, sažetak, znanstveni)


Naslov
Nucleophosmin cytoplasmic and nucleus immunohistochemical cell positivity of bone marrow biopsy sections in AML and MDS patients

Autori
Sučić, Mirna ; Ilić, Ivana ; Dotlić ; Snježana ; Labar, Boris ; Ljubić, Nives ; Županić Krmek, Dubravka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Leukemia Research, Suppl.37, 2013 / Bennett, John M ; Feldman, Eric J - Oxford, UK : Elsevier, 2013, S135-s136

Skup
12th International Symposium on Myelodisplastic Syndromes (MDS)

Mjesto i datum
Berlin, Njemačka, 08-11.05.2013

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Nucleophosmin; acute myeloid leukemia; myelodisplasia

Sažetak
Background Nucleophosmin (NPM) is phosphoprotein in nucleolus and shuttles continuously between nucleus and cytoplasm. Major role of NPM is to mediate nuclear export of ribosome components to the cytoplasm and to control centrosome duplication and it also interacts with oncosupressors p53 and p19Arf thus controlling cell proliferation and apoptosis. NPM mutations are involved in pathogenesis of some AML cases. Introduction The abnormal mutated NPM1 protein shows an aberrant cytoplasmic localization in leukemic cells (NPMc), whereas the wild-type protein is mainly located in the nucleolus and on the nuclear membrane. Approximately one-third of cases of denovo acute myeloblastic leukemia (AML) carry mutations in the c-terminal region of NPM (NPM1) gene. NPM1 mutations are rare in myelodisplasia (MDS) but in some reports NPM mutations and NPMc cell bone marrow cell positivity were also found in MDS. Purpose Aim of the study were to analyze cell NPM immunohistochemical expression in nuclei (NPMn) and cytoplasm (NPMc) in bone marrow (BM) sections of 7 patients with de novo AML and 9 MDS patients. Materials and Methods Immunohistochemical procedures were performed on paraffin sections cut from B5-fixed/EDTA-decalcified BM biopsies. Sections were subjected to micro waving/antigen retrieval, pH9 and immunostained with anti-NPM monoclonal antibody (clone 376) using immuno alkaline phosphatase technique. NPMc and NPMn cell positivity between AML and MDS patients were statistically compared with Mann-Whitney and Chi-square tests. From patients informed consent was obtained. Results NPMn cell positivity was seen in 6/7 AML and in 8/9 MDS patients. NPMc was detected in 5/7 AML and in 8 out of 9 MDS patients. Percentages of NPMn of BM cells were higher in MDS than in AML patients with borderline statistical significance. NPMn immunoexpression above 30% of BM positive cells was more frequently (with statistical significance) observed in MDS than in AML patients (p< 0.05). Conclusions Higher NPMn cell positivity (above 30% of nucleated BM cells) in MDS is probably linked to higher number of normal BM cells in MDS. Lower NPMn cell positivity in AML is maybe associated to higher number of leukemic cells and also to NPM functional loss in progression and transformation of MDS to AML. NPMc was also observed in most of our AML and MDS patients. This finding is in a concordance with some recent reports also implicating that that NPM1 mutations may be also seen in MDS, but further studies are needed to analyze correlation of NPMc cell positivity and NPM1 mutations in MDS patients

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti, Farmacija



POVEZANOST RADA


Projekt / tema
129-0000000-3385 - Citološki pokazatelji proliferacije stanica (Mirna Sučić, )

Ustanove
Farmaceutsko-biokemijski fakultet, Zagreb,
Medicinski fakultet, Zagreb,
Klinička bolnica "Sveti Duh"

Citiraj ovu publikaciju

Sučić, Mirna; Ilić, Ivana; Dotlić; Snježana; Labar, Boris; Ljubić, Nives; Županić Krmek, Dubravka
Nucleophosmin cytoplasmic and nucleus immunohistochemical cell positivity of bone marrow biopsy sections in AML and MDS patients // Leukemia Research, Suppl.37, 2013 / Bennett, John M ; Feldman, Eric J (ur.).
Oxford, UK: Elsevier, 2013. str. S135-s136 (poster, međunarodna recenzija, sažetak, znanstveni)
Sučić, M., Ilić, I., Dotlić, Snježana, Labar, B., Ljubić, N. & Županić Krmek, D. (2013) Nucleophosmin cytoplasmic and nucleus immunohistochemical cell positivity of bone marrow biopsy sections in AML and MDS patients. U: Bennett, J. & Feldman, E. (ur.)Leukemia Research, Suppl.37, 2013.
@article{article, year = {2013}, pages = {S135-s136}, keywords = {nucleophosmin, acute myeloid leukemia, myelodisplasia}, title = {Nucleophosmin cytoplasmic and nucleus immunohistochemical cell positivity of bone marrow biopsy sections in AML and MDS patients}, keyword = {nucleophosmin, acute myeloid leukemia, myelodisplasia}, publisher = {Elsevier}, publisherplace = {Berlin, Njema\v{c}ka} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE