engleski

Barrier function, cytokine and natural moisturizing factor levels after in vivo tandem repeated irritation in healthy and atopic individuals

Introduction: The barrier function, cytokine and natural moisturizing factor (NMF) levels after in vivo tandem irritation in atopic skin have not been studied so far. Material and methods: We therefore investigated the barrier function parameteres, levels of primary keratinocyte-derived cytokine and NMF after repeated single and tandem exposure to surfactants (0.5% sodium lauryl sulfate ; SLS) and organic acids (2.0% acetic acid ; AcA) in atopic dermatitis patients and healthy controls with known filaggrin (FLG) genotype. The irritant response was monitored by visual scoring and repeated measurements and transepidrmal water loss (TEWL) and erythema ; additionally tape strips for NMF and cytokine analysis were collected from the irritant-exposed and non-exposed (control) skin sites. Results: Compared to the control group, repeated single (AcA/AcA and SLS/SLS) and tandem (AcA/SLS and SLS/AcA) irritation in the atopic dermatitis patients resulted in greater visual score and erythema (a*-value). In atopic skin, the TEWL increase compared to baseline (ΔTEWL) was more pronounced at all exposed fields and assessment time points. Furthermore, ΔTEWL after 96- hour exposure to AcA/SLS, SLS/AcA and SLS/SLS in the atopic dermatitis group was significantly higher compared to the controls. In both groups, repeated exposure to AcA/SLS, SLS/AcA and SLS/SLS, but not to AcA/AcA, resulted in significantly decreased stratum corneum levels of NMF, IL-1α and IL-1RA when compared to the non-exposed skin (control). Within the atopic dermatitis group no significant differences in the barrier function parameters and reduction of NMF levels between the FLG mutation carriers and non-carriers were found. Conclusion: Our results indicate that atopic dermatitis confers and enhanced reactivity to cumulative skin barrier damage by multiple sub-treshold irritant stimuli, irrespecitve of FLG genotype. Furthermore, to the best of our knowledge, we provide first evidence for reduced NMF levels in result of in vivo tandem repeated irritation in healthy and atopic skin.

stratum corneum; irritation; atopic dermatitis

nije evidentirano