engleski

Induction of apoptosis in the blue mussel Mytilus galloprovincialis by tri-n-butyltin chloride

Induction of apoptosis by TBT in gill tissue of the mussel Mytilus galloprovincialis was investigated. The terminal dUTP nick-end labeling technique (TUNEL) was used to detect cells displaying DNA fragmentation within gills structures. Genomic DNA fragmentation was detected as characteristically ladder-like pattern of DNA fragments induced by single injection of different doses of TBT (1-5 ľg/g ) below the mantle, directly into the pallial fluid, after 24 hours of incubation. DNA degradation of higher order DNA structure as well as reduced G0/G1 cell cycle region (the sub-G1 region) was detectable after 1.5 hours of TBT incubation. Presence of apoptotic cells in mussels' gills was indicated by the selective loss of G2/M cells concomitant with the appearance of cells with decreased DNA content in S and G0/G1 cell cycle regions. The effect of TBT on cell cycle in mussel gill was dose related and exposure time depending.The possible mechanism of induction of apoptosis in vivo in gill tissue of mussel treated with TBT is discussed.

DNA damage; apoptosis; tributyltin; mussel

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