engleski

Two fluorogenic substrates for purine-nucleoside phosphorylase, selective for mammalian and bacterial forms of the enzyme

Two non-typical nucleosides, 7-β-D-ribosyl-2, 6-diamino-8-azapurine and 8-β-D-ribosyl-2, 6-diamino-8-azapurine, have been found to exhibit moderately good, and selective, substrate properties towards calf and bacterial (E. coli) forms of purine-nucleoside phosphorylase (PNP). The former compound is effectively phosphorolysed by calf PNP, and the latter by PNP from E. coli. Both compounds are fluorescent with λmax ∼ 425-430 nm, but the reaction product, 2, 6-diamino-8-azapurine, emits in different spectral region (λmax ∼ 363 nm), with nearly 40% yield, providing strong fluorogenic effect at 350-360 nm.

fluorogenic substrates; 8-azapurines; nucleoside analogs; purine-nucleoside phosphorylase

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