engleski

Arabidopsis thaliana cytosolic seryl-tRNA synthetase: studying protein-protein interactions

Aminoacyl-tRNA synthetases (AARS) are essential cellular enzymes that play significant role in protein synthesis by charging amino acids to their cognate tRNAs. In recent years more evidence appeared showing involvement of these enzymes in diverse cellular functions beyond translation. Characterization of these non-canonical functions broadens our knowledge in functional proteomics. The studies of aaRS assemblies in plants are scarce. In search for cytosolic seryl-tRNA synthetase (SerRS) interacting protein partners in plant Arabidopsis thaliana, L. we conducted yeast- two hybrid (Y2H) and tandem affinity purification (TAP) screening assays. Potential interacting partners were identified either by DNA sequencing (Y2H) or mass spectrometry (TAP).

Arabidopsis thaliana; Seryl-tRNA synthetase; Yeast-two hybrid; Tandem affinity purification

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