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Principles and approaches for bioremediation of PCB-contaminated soils: a case study

Petrić, Ines; Hršak, Dubravka; Bru, David; Udikovic-Kolić, Nikolina; Martin-Laurent, Fabrice
Principles and approaches for bioremediation of PCB-contaminated soils: a case study // EUROSOIL 2012
Bari, Italy, 2012. (predavanje, međunarodna recenzija, sažetak, znanstveni)

Principles and approaches for bioremediation of PCB-contaminated soils: a case study

Petrić, Ines ; Hršak, Dubravka ; Bru, David ; Udikovic-Kolić, Nikolina ; Martin-Laurent, Fabrice

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

EUROSOIL 2012 / - Bari, Italy, 2012

EUROSOIL 2012, 4th International Congress of the European Confederation of Soil Science Societies (ECSSS)

Mjesto i datum
Bari, Italija, 02-06.07.2012

Vrsta sudjelovanja

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Polychlorinated biphenyls; soil; bioremediation; microbail community
(Polychlorinated biphenyls; soil; bioremediation; microbial community)

Widespread use of polychlorinated biphenyls (PCBs), recalcitrant and toxic compounds, yielded in the contamination of the environment. In Croatia, warfare activities in the 90s led to heavy PCBs contamination of several sites. Remediation of PCBs contaminated sites is essential to restore environmental quality. In this context, we report the principal steps that yielded in the implementation of PCB bioremediation assay. The first step was to isolate seed culture able to transform PCBs from the contaminated site in Zadar (Croatia). A mixed bacterial community (TSZ7), enriched using biphenyl as the only carbon source, showed high capacities to degrade a wide range of PCBs (56-60% degradation of the PCBs in liquid culture) [1]. Strain Z6, identified as Rhodococcus sp., was found as the active PCB-degrading member of the mixed community. Intermediary metabolites were identified using GC-MS analysis and single PCB congeners (2, 4, 4'- and 2, 2', 5, 5'- chlorobiphenyl). The second step was to select natural compounds such as carvone, tested as inducers of PCB degrading pathway, and soya lecithin, tested to enhance PCB bioavailability. The third step was to develop and monitor a small-scale bioremediation assay. Both biostimulation and bioaugmentation (inoculation with TSZ7 or Rhodococcus sp. Z6) strategies were tested, leading to the reduction of significant amount of PCBs [2]. The last step was to address the impact of bioremediation on the global indigenous microbial community and on the PCB-degrading functional community [2, 3]. These results gave new insights into processes following bioremediation practices and therefore could help to promote implementation of the bioremediation in restoration of PCB-contaminated sites.

Izvorni jezik

Znanstvena područja


Projekt / tema
098-0982934-2712 - Organski spojevi kao molekulski obilježivači antropogenog utjecaja na okoliš (Marijan Ahel, )

Institut "Ruđer Bošković", Zagreb