hrvatski

Jesu li životinje u Republici Hrvatskoj potencijalni rezervoari virusa hepatitisa E?

Hepatitis E (HE) je značajan javnozdravstveni problem s mogućim zoonotskim potencijalom. Uzročnik HE je RNK virus, koji pripada rodu Hepevirus, porodici Hepeviridae. Do danas su poznate četiri genetske skupine virusa hepatitisa E (HEV). Filogenetskom je analizom sojeva izdvojenih iz svinja utvrđena visoka genetska podudarnost sa sojevima izdvojenima iz ljudi. Dokazana je i statistički značajno viša HE seroprevalencija u ljudi koji dolaze u izravni kontakt sa svinjama. Temeljem hipoteze o većem riziku od pojave HE u pojedinim profesionalnim skupinama ljudi (veterinari i uzgajivači životinja), u Hrvatskom veterinarskom institutu je 2009. godine započeto istraživanje prisutnosti HEV metodom umrežene lančane reakcije polimerazom uz prethodnu reverznu transkripciju (nested RTPCR) u različitih vrsti domaćih i divljih životinja podrijetlom iz 17 županija Republike Hrvatske (RH).HEV je dokazan u domaćih svinja, neovisno o načinu držanja (veliki farmski ili dvorišni manji uzgoji), dobi i spolu u 7 županija (Bjelovarsko bilogorska, Brodsko posavska, Koprivničko križevačka, Osječko baranjska, Sisačko moslavačka, Virovitičko podravska i Vukovarsko srijemska) u prevalenciji od 29, 6% (95% CI 26, 3%-33, 2%). Najviša HEV prevalencija (57, 9% s 95%CI 47, 8-67, 3%) je utvrđena u domaćih svinja iz dvorišnih uzgoja Sisačko moslavačke županije. HEV je dokazan i u divljih svinja u 4 istraživane županije (Brodsko posavska, Sisačko moslavačka, Virovitičko podravska i Vukovarsko srijemska) u prevalenciji od 57, 9% (95%CI 47, 8-67, 3%). Dokazani sojevi virusa su podvrgnuti sekvencioniranju odsječka dužine 287bp unutar ORF-1 regije genoma koji kodira za metiltransferazu (HEV-MeT), te je utvrđena njihova genetska pripadnost skupini 3, podskupinama 3a i 3e. Genetička istovjetnost HEV sojeva izdvojenih iz domaćih i divljih svinja ukazuje na mogući prijenos virusa između ovih populacija. Usporedbom dobivenih rezultata sekvencioniranja s rezultatom sekvencioniranja autohtonog soja HEV izdvojenog iz čovjeka u RH utvrdili smo pripadnost istoj genetskog skupini 3, ali različitim genetskim podskupinama. To ukazuje na potrebu za daljnjim istraživanjem mogućih izvora i rezervoara infekcije HE u RH. Hepatitis E is an important health problem with a possible zoonotic potential. Hepatitis E virus (HEV)belongs to the genus Hepevirus, family Hepeviridae. Four HEV genotypes have been recognized so far. Genetic typing of swine HEV strains, indicate their close relationship to human HEV strains. Furthermore, a statistically higher seroprevalence has been detected in people that come in close contact with swine. Based on the hypothesis that some proffesional groups (veterinarians and animal breeders) could be at higher risk for HE infection, in 2009, the Croatian Veterinary Institute started an investigation by a nested RT-PCR protocol in regards to determine the viral prevalence of HEV in different animal species from 17 counties in Croatia. HEV was detected in domestic swine, independetely of their origin (large commercial farms, or small holdings), age or sex in 7 counties (Bjelovar Bilogora, Brod Posavina, Koprivnica Križevci, Osijek Baranja, Sisak Moslavina, Virovitica Podravina and Vukovar Srijem) with a prevalence of 29, 6% (95% CI 26, 3%-33, 2%). The highest viral prevalence (57, 9% ; 95%CI 47, 8-67, 3%) was found in domestic swine from small holdings in Sisak Moslavina County. HEV was also detected in wild boars from 4 counties included in the investigation (Brod Posavina, Sisak Moslavina, Virovitica Podravina and Vukovar Srijem) with a prevalence of 57, 9% (95%CI 47, 8-67, 3%). Furthermore, a fragment of the metil-transferase gene (HEV-MeT) of 287bp within the ORF-1 genome region of representative isolates was sequenced. Phylogenetic analysis showed that they are members of group 3, clustering into two separate subgroups (3a and 3e). Since identical HEV strains were found in domestic swine and wild boars, the possibility of viral transmission between these two populations is possible. Comparison of the obtained genetical results of strains delivered from domestic swine and wild boars with a strain delivered from a human HE case in Croatia shows that they all belong to group 3, however they cluster into different subgroups. We conclude that further investigation is needed to determine possible sources and reservoires of HEV infection in Croatia.

Hepatitis E virus; životinje; genetska tipizacija; Hrvatska

nije evidentirano

engleski

Are animals possible reservoires of hepatitis E in Croatia?

Hepatitis E (HE) je značajan javnozdravstveni problem s mogućim zoonotskim potencijalom. Uzročnik HE je RNK virus, koji pripada rodu Hepevirus, porodici Hepeviridae. Do danas su poznate četiri genetske skupine virusa hepatitisa E (HEV). Filogenetskom je analizom sojeva izdvojenih iz svinja utvrđena visoka genetska podudarnost sa sojevima izdvojenima iz ljudi. Dokazana je i statistički značajno viša HE seroprevalencija u ljudi koji dolaze u izravni kontakt sa svinjama. Temeljem hipoteze o većem riziku od pojave HE u pojedinim profesionalnim skupinama ljudi (veterinari i uzgajivači životinja), u Hrvatskom veterinarskom institutu je 2009. godine započeto istraživanje prisutnosti HEV metodom umrežene lančane reakcije polimerazom uz prethodnu reverznu transkripciju (nested RTPCR) u različitih vrsti domaćih i divljih životinja podrijetlom iz 17 županija Republike Hrvatske (RH).HEV je dokazan u domaćih svinja, neovisno o načinu držanja (veliki farmski ili dvorišni manji uzgoji), dobi i spolu u 7 županija (Bjelovarsko bilogorska, Brodsko posavska, Koprivničko križevačka, Osječko baranjska, Sisačko moslavačka, Virovitičko podravska i Vukovarsko srijemska) u prevalenciji od 29, 6% (95% CI 26, 3%-33, 2%). Najviša HEV prevalencija (57, 9% s 95%CI 47, 8-67, 3%) je utvrđena u domaćih svinja iz dvorišnih uzgoja Sisačko moslavačke županije. HEV je dokazan i u divljih svinja u 4 istraživane županije (Brodsko posavska, Sisačko moslavačka, Virovitičko podravska i Vukovarsko srijemska) u prevalenciji od 57, 9% (95%CI 47, 8-67, 3%). Dokazani sojevi virusa su podvrgnuti sekvencioniranju odsječka dužine 287bp unutar ORF-1 regije genoma koji kodira za metiltransferazu (HEV-MeT), te je utvrđena njihova genetska pripadnost skupini 3, podskupinama 3a i 3e. Genetička istovjetnost HEV sojeva izdvojenih iz domaćih i divljih svinja ukazuje na mogući prijenos virusa između ovih populacija. Usporedbom dobivenih rezultata sekvencioniranja s rezultatom sekvencioniranja autohtonog soja HEV izdvojenog iz čovjeka u RH utvrdili smo pripadnost istoj genetskog skupini 3, ali različitim genetskim podskupinama. To ukazuje na potrebu za daljnjim istraživanjem mogućih izvora i rezervoara infekcije HE u RH. Hepatitis E is an important health problem with a possible zoonotic potential. Hepatitis E virus (HEV)belongs to the genus Hepevirus, family Hepeviridae. Four HEV genotypes have been recognized so far. Genetic typing of swine HEV strains, indicate their close relationship to human HEV strains. Furthermore, a statistically higher seroprevalence has been detected in people that come in close contact with swine. Based on the hypothesis that some proffesional groups (veterinarians and animal breeders) could be at higher risk for HE infection, in 2009, the Croatian Veterinary Institute started an investigation by a nested RT-PCR protocol in regards to determine the viral prevalence of HEV in different animal species from 17 counties in Croatia. HEV was detected in domestic swine, independetely of their origin (large commercial farms, or small holdings), age or sex in 7 counties (Bjelovar Bilogora, Brod Posavina, Koprivnica Križevci, Osijek Baranja, Sisak Moslavina, Virovitica Podravina and Vukovar Srijem) with a prevalence of 29, 6% (95% CI 26, 3%-33, 2%). The highest viral prevalence (57, 9% ; 95%CI 47, 8-67, 3%) was found in domestic swine from small holdings in Sisak Moslavina County. HEV was also detected in wild boars from 4 counties included in the investigation (Brod Posavina, Sisak Moslavina, Virovitica Podravina and Vukovar Srijem) with a prevalence of 57, 9% (95%CI 47, 8-67, 3%). Furthermore, a fragment of the metil-transferase gene (HEV-MeT) of 287bp within the ORF-1 genome region of representative isolates was sequenced. Phylogenetic analysis showed that they are members of group 3, clustering into two separate subgroups (3a and 3e). Since identical HEV strains were found in domestic swine and wild boars, the possibility of viral transmission between these two populations is possible. Comparison of the obtained genetical results of strains delivered from domestic swine and wild boars with a strain delivered from a human HE case in Croatia shows that they all belong to group 3, however they cluster into different subgroups. We conclude that further investigation is needed to determine possible sources and reservoires of HEV infection in Croatia.

Hepatitis E virus; animals; genetic characterization; Croatia

nije evidentirano