engleski

The effect of air-drying on glycosidically bound volatiles from seasonally collected origano (Origanum vulgare ssp. hirtum) from Croatia

The glycosidically bound volatiles were isolated from fresh and air-dried origano by exhaustive percolation with ethyl acetate. After the purification of glycosidic fraction, the enzymatic hydrolysis with  -glucosidase was performed. The obtained aglycones were analysed by GC-MS. Eighteen compounds were identified. The seasonal variations of main aglycone contents from the fresh plant material were: thymoquinone (3.10-6.18 mg kg-1), benzyl alcohol (1.33-3.62 mg kg-1), 3, 5, 5-trimethyl-4-(3-hydroxy-1-buthenyl)-2-cyclohexen-1-on (0.51-3.35 mg kg-1), 2-phenyl ethanol (0.42-2.98 mg kg-1), eugenol (0.93-2.55 mg kg-1), thymol (0.70-1.40 mg kg-1) and carvacrol (0.88-1.40 mg kg-1). The season of collecting influenced the content and composition of the glycosidically bound volatiles of origano. Air-drying effected mostly the yield of these compounds.

Origanum vulgare L. ; essential oil ; seasonally variation

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