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Pivoting of microtubules around the spindle pole accelerates kinetochore capture


Kalinina, Iana; Nandi, Amitabha; Delivani, Petrina; Chacón, Mariola R.; Klemm, Anna H.; Ramunno-Johnson, Damien; Krull, Alexander; Lindner, Benjamin; Pavin, Nenad; Tolić-Nørrelykke, Iva M.
Pivoting of microtubules around the spindle pole accelerates kinetochore capture // Nature cell biology, 15 (2013), 82-87 doi:10.1038/ncb2640 (međunarodna recenzija, članak, znanstveni)


Naslov
Pivoting of microtubules around the spindle pole accelerates kinetochore capture

Autori
Kalinina, Iana ; Nandi, Amitabha ; Delivani, Petrina ; Chacón, Mariola R. ; Klemm, Anna H. ; Ramunno-Johnson, Damien ; Krull, Alexander ; Lindner, Benjamin ; Pavin, Nenad ; Tolić-Nørrelykke, Iva M.

Izvornik
Nature cell biology (1465-7392) 15 (2013); 82-87

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Kinetochore capture; metaphase; microtubule; microtubule assembly; microtubule pivoting motion; mitosis; polar microtubule; Schizosaccharomyces pombe; theoretical model

Sažetak
During cell division, spindle microtubules attach to chromosomes through kinetochores, protein complexes on the chromosome. The central question is how microtubules find kinetochores. According to the pioneering idea termed search- and-capture, numerous microtubules grow from a centrosome in all directions and by chance capture kinetochores. The efficiency of search-and-capture can be improved by a bias in microtubule growth towards the kinetochores, by nucleation of microtubules at the kinetochores and at spindle microtubules, by kinetochore movement, or by a combination of these processes. Here we show in fission yeast that kinetochores are captured by microtubules pivoting around the spindle pole, instead of growing towards the kinetochores. This pivoting motion of microtubules is random and independent of ATP-driven motor activity. By introducing a theoretical model, we show that the measured random movement of microtubules and kinetochores is sufficient to explain the process of kinetochore capture. Our theory predicts that the speed of capture depends mainly on how fast microtubules pivot, which was confirmed experimentally by speeding up and slowing down microtubule pivoting. Thus, pivoting motion allows microtubules to explore space laterally, as they search for targets such as kinetochores.

Izvorni jezik
Engleski

Znanstvena područja
Fizika, Biologija



POVEZANOST RADA


Projekt / tema
119-0982464-1253 - KSA kompjutorsko istraživanje repeticija višeg reda i duplikona u humanom genomu (Vladimir Paar, )

Ustanove
Prirodoslovno-matematički fakultet, Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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