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Comparison of Haemoglobin and Haematocrit Levels On Point of Care Blood Gas Analyzer and Automated Haematology Analyzer

Background: The aim of this study was to compare haemoglobin and haematocrit levels measured as point of care testing and measurements done by automated haematology analyzer. Materials and methods: In this prospective study, haemoglobin and haematocrit levels were measured in 126 consecutive paired arterial or capillary samples using a point of care blood gas analyzer and automated haematology analyzer. Pearson correlation coefficient and Passing-Bablok regression were calculated for the data comparison. Results: Mean (SD) level of haemoglobin measured on blood gas analyzer was 135.59 g/L (26.48) and measured on automated haematology analyzer 118.84 g/L (25.59). The mean difference between the blood gas analyzer and automated haematology analyzer was -16.75 g/L (P < 0.001). Mean (SD) level of haematocrit measured on blood gas analyzer was 0.399 L/L (0.078) and measured on automated haematology analyzer 0.356 L/L (0.071), respectively. The mean difference between the blood gas analyzer and automated haematology analyzer was -0.043 L/L (P < 0.001). Comparison of the results of two devices yielded correlation coefficients r = 0.98 (P < 0.001) for haemoglobin and r = 0.75 (P < 0.001) for haematocrit. Conclusions: Although the correlations between the measurements were significant, blood gas analyzer has shown unacceptable agreement with automated haematology analyzer. It overestimates haemoglobin and haematocrit values, which is not clinically acceptable.

point–of-care; blood gas analyzer; haematology analyzer; haemoglobin; haematocrit

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