engleski

Safety assessment and antioxidant activity of sodium copper chlorophyllin

Sodium copper chlorophyllin (CHL) is a semi-synthetic mixture of water-soluble sodium copper salts derived from chlorophyll that is widely used as a colour additive in foods, drugs, cosmetics and as a dietary supplement. Present study aimed to investigate the toxicological profile of CHL by measuring its cyto/genotoxic potential in human peripheral blood lymphocytes (HPBLs) in different concentrations (0.1 to 100 µg/ml) and lengths of time (4 and 24 h). After the treatment, genotoxic activity was measured with the comet and cytokinesis-block micronucleus (CBMN) assays. Moreover, free radical scavenging capacity/antioxidant activity of CHL was determined with DPPH assay. Results showed that CHL in tested concentrations had no effect on HPBLs viability regardless of exposure time. CHL in concentration range tested did not cause DNA damage that could be detected with the comet assay and also CHL did not induce statistically significant difference in either CBMN assay parameter compared to untreated control. Additionally, results revealed that CHL is an effective DPPH• scavenger with the concentration-dependent radical scavenging ability. By comparing the antioxidant activity of CHL with some of well-known and powerful antioxidants as Trolox, ascorbic acid and buthylated hydroxytoluene (BHT) the DPPH• free radical scavenging capacity of CHL was about 1.6 and 1.9 fold lower than of ascorbic acid and Trolox, respectively, and 4.3 fold higher than of BHT. Taken together, we have shown that CHL has no impact on cell viability and genome damage in the concentration range tested and in that way is safe from the aspect of cyto/genotoxicity. Since CHL also displayed powerful radical scavenging ability it could be a perfect candidate as a novel antioxidant.

Chlorophyllin; Food additive; Antioxidants; Lymphocytes

nije evidentirano