engleski

3D Culture of mouse neuronal stem cells in calcium alginate beads

One of the biggest challenges in modern medicine is the treatment of neuronal diseases and nerve injury repair due to incapability of the neural tissue to self-renew. Regenerative medicine and stem cell technology holds tremendous potential to treat a wide range of such medical problems. Although there is substantial work done with two dimensional cultures used for neural differentiation and proliferation protocols, surrounding environment and three dimensional culturing are important candidates to enhance cell proliferation and differentiation to desired cell lineage. Alginate hydrogel is a polysaccharide biocompatible with human tissue and it has appropriate physicochemical characteristics. Its gel-forming property is achieved through a cross- linking with calcium ions. Neural stem cells from the brain cortex of 14 days old mouse embryos were isolated and encapsulated in soft alginate hydrogel beads in concentration of 2 million cells per ml of 1% alginate. Cells were cultured following established neural proliferation protocols. On 7th day following encapsulation, live/dead test was performed. Confocal microscopy showed that cells were both viable and formed clusters. Alginate hydrogel was a good candidate for encapsulation and culturing of neuronal stem cells improving their proliferation and viability. Obtained results opened opportunities for further research on therapeutic treatment of neural tissue injuries

neural stem cells; alginate hydrogel

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