engleski

Clinical cytogenetics and toxogenetics with the Common aim : risk assessment of chemical mutagens

According to recent findings of DNA adducts formed after exposure to chemical mutagens it is suggested that certain parts of the DNA molecule, due to its structure, express higher fragility. In our study this speculation was investigated with well known mutagen and carcinogen, vinyl chloride (VCM). In a population occupationally exposed to VCM with elevated frequencies of chromosome aberrations the genome was analysed by G-banding. Clustering of chromosomes, chromatid braeks and deletions confirmed the non-random distribution of DNA damages. The most frequent damages were detected on bands: 1p13, 1q21, 1q23, 1q32, 3p21, 3q21, 5q31, 7p13, 9p21, 10q24, 11q23, 13q32. Very often the detected breakpoints coinceded with those detected in lymphoproliferative disorders and showed correlation with marked relative lymphocytosis detected in examinees. Methods of clinical cytogenetics and toxogenetics together offer potent tool in evaluating results that can be used for early detection of possible health risk and selecting biomarkers for biomonitoring of population exposed to known or suspected mutagens.

biomonitoring; VCM; occupational exposure; G-banding

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