CHARACTERIZATION OF THREE MAJOR CYTOKINES: IL1, TNF1 AND TNF2 IN REARED ATLANTIC BLUEFIN TUNA THUNNUS THYNNUS (CROSBI ID 600288)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Lepen Pleić, Ivana ; Secombes, Chris ; Bird, Steven ; Mladineo, Ivona
engleski
CHARACTERIZATION OF THREE MAJOR CYTOKINES: IL1, TNF1 AND TNF2 IN REARED ATLANTIC BLUEFIN TUNA THUNNUS THYNNUS
Atlantic bluefin tuna (Thunnus thynnus) (BFT) is of great economic significance for Croatian aquaculture and therefore it is necessary to ensure optimal and sustainable conditions during its farming process. Intensive culture of BFT is limited by infectious diseases that, beside the stress, cause heavy losses negatively affecting an individual's immunocompetence and growth performance. However, to date there are no reports of cloning and expression analysis of any major immunity gene of BFT, therefore in this study we have characterized the first cytokine molecules in Atlantic bluefin tuna, through: 1) Isolation of full-length cDNA and gene sequences of Atlantic BFT TNFα1, TNFα2, IL1β and their comparison to known sequences in other vertebrates, especially teleost fish ; 2) Designing of three- dimensional models for three BFT cytokines based on homology modeling ; 3) Quantification of in vivo tissue specific expression of chosen cytokines in reared BFT over the duration (two years) of the farming process, in order to evaluate their importance as a putative health biomarkers within tuna aquaculture. Understanding of how the innate immunity response is functioning in this bluefin species will allow us to study immune regulation more detailed.
tuna; immunity; cytokines; cloning; expression
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Podaci o prilogu
268-268.
2013.
objavljeno
Podaci o matičnoj publikaciji
Abstract book of the 16th International Conference of Fish and Shellfish Diseases
Garcia, Jose
Helsinki: Nykypaino
Podaci o skupu
16th International Conference of Fish and Shellfish Diseases
poster
02.09.2013-06.09.2013
Tampere, Finska