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The effects of guanylat cyclase A agonists on the bradykinin signaling pathway after ischemic mouse brain injury (CROSBI ID 600074)

Prilog sa skupa u časopisu | sažetak izlaganja sa skupa | međunarodna recenzija

Dobrivojević, Marina ; Špiranec, Katarina ; Erjavec, Igor ; Gorup, Dunja ; Habek, Nikola ; Hirsch, Jochen R ; Forssmann, Wolf-Georg ; Schlatter, Eberhard ; Sinđić, Aleksandra The effects of guanylat cyclase A agonists on the bradykinin signaling pathway after ischemic mouse brain injury // Periodicum biologorum. 2013. str. 25-25

Podaci o odgovornosti

Dobrivojević, Marina ; Špiranec, Katarina ; Erjavec, Igor ; Gorup, Dunja ; Habek, Nikola ; Hirsch, Jochen R ; Forssmann, Wolf-Georg ; Schlatter, Eberhard ; Sinđić, Aleksandra

engleski

The effects of guanylat cyclase A agonists on the bradykinin signaling pathway after ischemic mouse brain injury

Bradykinin is involved in the formation of cerebral edema after ischemic brain injury increasing the size of brain. Furthmore, it is known that natriuretic peptides are involved in decreasing cerebral edema via still unknown mechanisms. First, we examined the effects of natriuretic peptides and bradykinin in vitro in HEK-293 cells, primary isolated neurons and astrocytes using the whole cell patch clamp technique and by measuring the intracellular calcium concentration. In a mouse model of ischemic brain injury, we measured the size of the ischemic lesion and edema using microCT. In HEK- 293 cells, ligands of GC-A, but not GC-B, inhibit the bradykinin signaling pathway by activating RGS protein which is responsible for inactivation of G coupled protein. Our preliminary results show that the same inhibition exists in primary isolated mouse neurons. In in vivo experiments, when urodilatin, agonist of GC-A was applied, no brain lesion was detected by microCT scanning. When only bradykinin was applied, brain damage increased. Applying a combination of natriuretic peptides with bradykinin the size of the lesion and the brain edema decreased. The results indicated the existence of an endogenous antagonist of the bradykinin signaling pathway and a possible protective role of natriuretic peptides during stroke.

bradykinin; guanylat cyclase A; mouse; ischemic injury

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Podaci o prilogu

25-25.

2013.

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objavljeno

Podaci o matičnoj publikaciji

Periodicum biologorum

Zagreb: Croatian Society of Natural Sciences

0031-5362

Podaci o skupu

Treći Kongres Hrvatskog društva fiziologa i prvi Regionalni kongres fizioloških društava

predavanje

13.09.2013-15.09.2013

Rijeka, Hrvatska

Povezanost rada

Temeljne medicinske znanosti

Indeksiranost