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Pregled bibliografske jedinice broj: 643554

Epigenetic characterization of chromatin in cycling cells of pedunculate oak, Quercus robur L.


Vičić, Vedrana; Barišić, Darko; Horvat, Tomislav; Biruš, Ivan; Zoldoš, Vlatka
Epigenetic characterization of chromatin in cycling cells of pedunculate oak, Quercus robur L. // Tree genetics & genomes, 9 (2013), 1247-1256 doi:10.1007/s11295-013-0632-x (međunarodna recenzija, članak, znanstveni)


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Naslov
Epigenetic characterization of chromatin in cycling cells of pedunculate oak, Quercus robur L.

Autori
Vičić, Vedrana ; Barišić, Darko ; Horvat, Tomislav ; Biruš, Ivan ; Zoldoš, Vlatka

Izvornik
Tree genetics & genomes (1614-2942) 9 (2013); 1247-1256

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
interphase chromatin; chromocenters; DNA methylation; epigenetics; histone acetylation; histone methylation; Quercus robur

Sažetak
Cycling cells of Quercus robur have a simple nuclear organization where most of the heterochromatin is visible as DAPI-positive chromocenters, which correspond to DAPI bands at the (peri)centromeric region of each of the 24 chromosomes of the oak complement. Immunofluorescence using 5-mC revealed dispersed distribution of the signal throughout the interphase nucleus/chromosomes without enrichment within DAPI-positive chromocenters/bands, suggesting that DNA methylation was not restricted to constitutive heterochromatin, but was associated with both euchromatic and heterochromatic domains. While H3K9ac exhibited typical euchromatin-specific distribution, the distributional pattern of histone methylation marks H3K9me1, H3K27me2, and H3K4me3 showed some specificity. The H3K9me1 and H3K27me2, both heterochromatin-associated marks, were not restricted to chromocenters, but showed additional dispersed distribution within euchromatin, while H3K27me2 mark also clustered in foci that did not co-localize with chromocenters. Surprisingly, even though H3K4me3 was distributed in the entire chromatin, many chromocenters were enriched with this euchromatin-specific modification. We discuss the distribution of the epigenetic marks in the context of the genome composition and lifestyle of Q. robur.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekt / tema
119-1191196-1224 - Dinamika kromatina i plastičnost genoma (Vlatka Zoldoš, )

Ustanove
Prirodoslovno-matematički fakultet, Zagreb

Citiraj ovu publikaciju

Vičić, Vedrana; Barišić, Darko; Horvat, Tomislav; Biruš, Ivan; Zoldoš, Vlatka
Epigenetic characterization of chromatin in cycling cells of pedunculate oak, Quercus robur L. // Tree genetics & genomes, 9 (2013), 1247-1256 doi:10.1007/s11295-013-0632-x (međunarodna recenzija, članak, znanstveni)
Vičić, V., Barišić, D., Horvat, T., Biruš, I. & Zoldoš, V. (2013) Epigenetic characterization of chromatin in cycling cells of pedunculate oak, Quercus robur L.. Tree genetics & genomes, 9, 1247-1256 doi:10.1007/s11295-013-0632-x.
@article{article, year = {2013}, pages = {1247-1256}, DOI = {10.1007/s11295-013-0632-x}, keywords = {interphase chromatin, chromocenters, DNA methylation, epigenetics, histone acetylation, histone methylation, Quercus robur}, journal = {Tree genetics and genomes}, doi = {10.1007/s11295-013-0632-x}, volume = {9}, issn = {1614-2942}, title = {Epigenetic characterization of chromatin in cycling cells of pedunculate oak, Quercus robur L.}, keyword = {interphase chromatin, chromocenters, DNA methylation, epigenetics, histone acetylation, histone methylation, Quercus robur} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


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