Beneficial effect of polyphenol quercetin against oxidative injury in the culture of P19 neurons is mediated by the prevention of caspase-independent apoptosis (CROSBI ID 599970)
Prilog sa skupa u časopisu | sažetak izlaganja sa skupa | domaća recenzija
Podaci o odgovornosti
Jazvinšćak Jembrek, Maja ; Vuković, Lidija ; Radovanović, Vedrana ; Erhardt, Julija ; Oršolić, Nada
engleski
Beneficial effect of polyphenol quercetin against oxidative injury in the culture of P19 neurons is mediated by the prevention of caspase-independent apoptosis
Introduction: Neuronal loss is a key observation of neurodegeneration implicated in the dysfunctions of the mammalian brain in physiological aging and numerous diseases and injuries. It is hypothesized that a dietary polyphenol supplementation could be an effective therapeutic strategy in minimizing the undesirable neuronal death. Materials and methods: Effects of ubiquitous flavonoid quercetin on neuronal death induced by exposure to 150 microM hydrogen peroxide (H2O2) for 24 hours were studied in the culture of P19 neurons. Reverse transcriptase PCR and western blot analysis were used to monitor changes in Bcl-2, Bax and PARP expression following H2O2 treatment. Changes in nuclear condensation were observed by Hoechst staining, while activities of key apoptotic markers caspase-3 and -7, and lactate dehydrogenase (LDH) activity were performed using commercially available assays (Promega). Results: Exposure to H2O2 decreased neuronal viability without changes in plasma membrane integrity, induced changes in chromatin condensation, slightly decreased Bcl-2 expression and moderately increased caspase-3/7 activity. Moreover, H2O2 induced strong PARP overexpression without PARP cleavage, altogether indicating a programmed type of cell death distinct from classical apoptosis. Presence of quercetin attenuated the toxic effects of H2O2 by preventing chromatin condensation and H2O2-induced changes in caspase activity, as well as changes in Bcl-2 and PARP expression. Conclusion: The obtained results suggest that the neuroprotective effect of polyphenol quercetin is related to its ability to prevent caspase-independent, PARP-dependent programmed cell death cascade. Hence, beneficial effects of quercetin might be assumed for the prevention of oxidative-stress driven neuronal loss in human aging and neurodegenerative diseases.
quercetin; hydrogen peroxide; P19 neurons; caspase-independent apoptosis; PARP expression
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Podaci o prilogu
76-76.
2013.
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objavljeno
Podaci o matičnoj publikaciji
Periodicum biologorum
Branko Vitale
Zagreb: Hrvatsko prirodoslovno društvo ; Institut Ruđer Bošković ; Laser Plus
0031-5362
Podaci o skupu
7th Croatian congress of pharmacology with international participation
poster
18.09.2013-21.09.2013
Zagreb, Hrvatska