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Pregled bibliografske jedinice broj: 643237

In silico optimization and low structured kinetic model of Poly[(R)-3-hydroxybutyrate] synthesis by Cupriavidus necator DSM 545 by fed-batch cultivation on glycerol


Vrana Špoljarić, Ivna; Lopar, Markan; Koller, Martin; Muhr, Alexander; Salerno, Anna; Reiterer, Angelika; Horvat, Predrag
In silico optimization and low structured kinetic model of Poly[(R)-3-hydroxybutyrate] synthesis by Cupriavidus necator DSM 545 by fed-batch cultivation on glycerol // Journal of biotechnology, 168 (2013), 625-635 doi:10.1016/j.jbiotec.2013.08.019 (međunarodna recenzija, članak, znanstveni)


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Naslov
In silico optimization and low structured kinetic model of Poly[(R)-3-hydroxybutyrate] synthesis by Cupriavidus necator DSM 545 by fed-batch cultivation on glycerol

Autori
Vrana Špoljarić, Ivna ; Lopar, Markan ; Koller, Martin ; Muhr, Alexander ; Salerno, Anna ; Reiterer, Angelika ; Horvat, Predrag

Izvornik
Journal of biotechnology (0168-1656) 168 (2013); 625-635

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Cupriavidus necator; Glycerol; Mathematical modeling; Optimization; PHB

Sažetak
Glycerol was utilized by Cupriavidus necator DSM 545 for production of poly-3-hydroxybutyrate (PHB) in fed-batch fermentation. Maximal specific growth rates (0.12 and 0.3 h−1) and maximal specific non-growth PHB production rate (0.16 g g−1 h−1) were determined from two experiments (inocula from exponential and stationary phase). Saturation constants for nitrogen (0.107 and 0.016 g L−1), glycerol (0.05 g L−1), non-growth related PHB synthesis (0.011 g L−1) and nitrogen/PHB related inhibition constant (0.405 g L−1), were estimated. Five relations for specific growth rate were tested using mathematical models. In silico performed optimization procedures (varied glycerol/nitrogen ratio and feeding) has resulted in a PHB content of 70.9%, shorter cultivation time (23 h) and better PHB yield (0.347 g g−1). Initial concentration of biomass 16.8 g L−1 and glycerol concentration in broth between 3 and 5 g L−1 were decisive factors for increasing of productivity.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija



POVEZANOST RADA


Ustanove:
Prehrambeno-biotehnološki fakultet, Zagreb

Profili:

Avatar Url Predrag Horvat (autor)

Avatar Url Ivna Vrana Špoljarić (autor)

Citiraj ovu publikaciju

Vrana Špoljarić, Ivna; Lopar, Markan; Koller, Martin; Muhr, Alexander; Salerno, Anna; Reiterer, Angelika; Horvat, Predrag
In silico optimization and low structured kinetic model of Poly[(R)-3-hydroxybutyrate] synthesis by Cupriavidus necator DSM 545 by fed-batch cultivation on glycerol // Journal of biotechnology, 168 (2013), 625-635 doi:10.1016/j.jbiotec.2013.08.019 (međunarodna recenzija, članak, znanstveni)
Vrana Špoljarić, I., Lopar, M., Koller, M., Muhr, A., Salerno, A., Reiterer, A. & Horvat, P. (2013) In silico optimization and low structured kinetic model of Poly[(R)-3-hydroxybutyrate] synthesis by Cupriavidus necator DSM 545 by fed-batch cultivation on glycerol. Journal of biotechnology, 168, 625-635 doi:10.1016/j.jbiotec.2013.08.019.
@article{article, year = {2013}, pages = {625-635}, DOI = {10.1016/j.jbiotec.2013.08.019}, keywords = {Cupriavidus necator, Glycerol, Mathematical modeling, Optimization, PHB}, journal = {Journal of biotechnology}, doi = {10.1016/j.jbiotec.2013.08.019}, volume = {168}, issn = {0168-1656}, title = {In silico optimization and low structured kinetic model of Poly[(R)-3-hydroxybutyrate] synthesis by Cupriavidus necator DSM 545 by fed-batch cultivation on glycerol}, keyword = {Cupriavidus necator, Glycerol, Mathematical modeling, Optimization, PHB} }
@article{article, year = {2013}, pages = {625-635}, DOI = {10.1016/j.jbiotec.2013.08.019}, keywords = {Cupriavidus necator, Glycerol, Mathematical modeling, Optimization, PHB}, journal = {Journal of biotechnology}, doi = {10.1016/j.jbiotec.2013.08.019}, volume = {168}, issn = {0168-1656}, title = {In silico optimization and low structured kinetic model of Poly[(R)-3-hydroxybutyrate] synthesis by Cupriavidus necator DSM 545 by fed-batch cultivation on glycerol}, keyword = {Cupriavidus necator, Glycerol, Mathematical modeling, Optimization, PHB} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


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