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Pregled bibliografske jedinice broj: 643112

Structural analysis of brain ganglioside acetylation patterns in mice with altered ganglioside biosynthesis


Mlinac, Kristina; Fabris, Dragana; Vukelić, Željka; Rožman, Marko; Heffer, Marija; Kalanj Bognar, Svjetlana
Structural analysis of brain ganglioside acetylation patterns in mice with altered ganglioside biosynthesis // Carbohydrate research, 382 (2013), 1-8 doi:10.1016/j.carres.2013.09.007 (međunarodna recenzija, članak, znanstveni)


Naslov
Structural analysis of brain ganglioside acetylation patterns in mice with altered ganglioside biosynthesis

Autori
Mlinac, Kristina ; Fabris, Dragana ; Vukelić, Željka ; Rožman, Marko ; Heffer, Marija ; Kalanj Bognar, Svjetlana

Izvornik
Carbohydrate research (0008-6215) 382 (2013); 1-8

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Brain gangliosides; mass spectrometric characterization; O-acetylated ganglioside species; O-acetylation patterns; St8sia1 null and B4galnt1 null mice

Sažetak
Gangliosides are sialylated membrane glycosphingolipids especially abundant in mammalian brain tissue. Sialic acid O-acetylation is one of the most common structural modifications of gangliosides which considerably influences their chemical properties. In this study, gangliosides extracted from brain tissue of mice with altered ganglioside biosynthesis (St8sia1 null and B4galnt1 null mice) were structurally characterized and their acetylation pattern was analyzed. Extracted native and alkali-treated gangliosides were resolved by high performance thin layer chromatography. Ganglioside mixtures as well as separated individual ganglioside fractions were further analyzed by tandem mass spectrometry. Several O-acetylated brain ganglioside species were found in knockout mice, not present in the wild-type mice. To the best of our knowledge this is the first report on the presence of O-acetylated GD1a in St8sia1 null mice and O-acetylated GM3 species in B4galnt1 null mice. In addition, much higher diversity of abnormally accumulated brain ganglioside species regarding the structure of ceramide portion was observed in knockout versus wild-type mice. Obtained findings indicate that the diversity of brain ganglioside structures as well as acetylation patterns in mice with altered ganglioside biosynthesis, is even higher than previously reported. Further investigation is needed in order to explore the effects of acetylation on ganglioside interactions with other molecules and consequently the physiological role of acetylated ganglioside species.

Izvorni jezik
Engleski

Znanstvena područja
Kemija



POVEZANOST RADA


Projekt / tema
098-0982915-2945 - Spektroskopija, kemijska svojstva i reakcije biološki aktivnih molekula (Branka Kovač, )
108-1081870-1877 - Uloga membranskih lipida u moždanom razvitku, starenju i neurodegeneraciji (Svjetlana Kalanj-Bognar, )
108-1081870-2415 - Strukturno-funkcionalna glikolipidomika moždanog razvitka i maligne alteracije (Željka Vukelić, )
219-0061194-2158 - Uloga lipidnih splavi i glikokonjugata u razvoju i regeneraciji živčanog sustava (Marija Heffer, )

Ustanove
Institut "Ruđer Bošković", Zagreb,
Medicinski fakultet, Zagreb,
Medicinski fakultet, Osijek

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Uključenost u ostale bibliografske baze podataka:


  • AGRICOLA
  • BIOSIS Previews (Biological Abstracts)
  • EMBASE (Excerpta Medica)
  • MEDLINE


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