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Pregled bibliografske jedinice broj: 642454

INFULENCE OF LED POLYMERISATION LIGHT FROM DENTAL CURING UNIT ON CONTINOUS CELL CULTURE OF FIBROBLASTS


Trosic, Ivancica; Pavicic, Ivan; Ivanisevic Malcic, Ana; Jukic Krmek, Silvana;
INFULENCE OF LED POLYMERISATION LIGHT FROM DENTAL CURING UNIT ON CONTINOUS CELL CULTURE OF FIBROBLASTS // 40th Annual Meeting of the European Radiation Research Society Book of Abstracts
Dublin, Irska, 2013. (poster, međunarodna recenzija, sažetak, znanstveni)


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Naslov
INFULENCE OF LED POLYMERISATION LIGHT FROM DENTAL CURING UNIT ON CONTINOUS CELL CULTURE OF FIBROBLASTS

Autori
Trosic, Ivancica ; Pavicic, Ivan ; Ivanisevic Malcic, Ana ; Jukic Krmek, Silvana ;

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
40th Annual Meeting of the European Radiation Research Society Book of Abstracts / - , 2013

Skup
40th Annual Meeting of the European Radiation Research Society

Mjesto i datum
Dublin, Irska, 1-5.09.2013

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Bluephase effects; LED light; fibroblasts

Sažetak
Objective: The aim of this study was to evaluate the effect of Bluephase light emitting diode (LED) light on cell viability, colony-forming ability and proliferation in V79 cell culture and to determine how much the temperature of the nutrient medium rose. Methods: The investigation included a low (L), soft start (S) and high (H) illumination mode for 20, 40 and 80 seconds. The viability was determined by the trypan blue exclusion test, colony-forming ability by counting colonies 7 days after exposure and cell proliferation by the cell counts on 5 post-exposure days. The temperature change during illumination was recorded (0.1˚C sensitivity). Results: In each experimental condition, 90-95% of the cells were viable, which was in the same range as the controls. Colony-forming ability was not found to be significantly lower (P<.05). A significant decrease in proliferation was recorded on the 4th post-exposure day with S and H irrespective of time, on the 3rd day with S for 80 s and H for 40 and 80 s, and with S and H for 80 s on the 2 nd day (P<.05).The temperature rise was significant with S (P<.05) and H (P<.05), irrespective of exposure duration. Conclusion: Dependent on total energy density, LED blue light affects the mitotic activity of cells in its path to a certain extent. Altered mitotic activity was not noted with illumination at the low power mode (intensity of 421.7 ±1.1 mW/cm 2 ). The greatest temperature rise was 8.3 ˚C and occurred at the highest intensity and exposure duration.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti



POVEZANOST RADA


Projekti:
022-0222411-2406 - BIOLOŠKI POKAZATELJI DJELOVANJA ELEKTROMAGNETSKOG NEIONIZIRAJUĆEG ZRAČENJA (Trošić, Ivančica, MZOS ) ( POIROT)
065-0650445-0434 - Epidemiološki aspekti endodoncije u Republici Hrvatskoj (Krmek, Silvana, MZOS ) ( POIROT)

Ustanove:
Institut za medicinska istraživanja i medicinu rada, Zagreb


Citiraj ovu publikaciju

Trosic, Ivancica; Pavicic, Ivan; Ivanisevic Malcic, Ana; Jukic Krmek, Silvana;
INFULENCE OF LED POLYMERISATION LIGHT FROM DENTAL CURING UNIT ON CONTINOUS CELL CULTURE OF FIBROBLASTS // 40th Annual Meeting of the European Radiation Research Society Book of Abstracts
Dublin, Irska, 2013. (poster, međunarodna recenzija, sažetak, znanstveni)
Trosic, I., Pavicic, I., Ivanisevic Malcic, A., Jukic Krmek, S. & (2013) INFULENCE OF LED POLYMERISATION LIGHT FROM DENTAL CURING UNIT ON CONTINOUS CELL CULTURE OF FIBROBLASTS. U: 40th Annual Meeting of the European Radiation Research Society Book of Abstracts.
@article{article, year = {2013}, keywords = {Bluephase effects, LED light, fibroblasts}, title = {INFULENCE OF LED POLYMERISATION LIGHT FROM DENTAL CURING UNIT ON CONTINOUS CELL CULTURE OF FIBROBLASTS}, keyword = {Bluephase effects, LED light, fibroblasts}, publisherplace = {Dublin, Irska} }
@article{article, year = {2013}, keywords = {Bluephase effects, LED light, fibroblasts}, title = {INFULENCE OF LED POLYMERISATION LIGHT FROM DENTAL CURING UNIT ON CONTINOUS CELL CULTURE OF FIBROBLASTS}, keyword = {Bluephase effects, LED light, fibroblasts}, publisherplace = {Dublin, Irska} }




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