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Pregled bibliografske jedinice broj: 641620

The effect of glucagon and cyclic adenosine monophosphate on acute liver damage induced by acetaminophen


Kelava, Tomislav; Ćavar, Ivan; Vukojević, Katarina; Saraga-Babić, Mirna; Ćulo, Filip
The effect of glucagon and cyclic adenosine monophosphate on acute liver damage induced by acetaminophen // Histology and histopathology, 28 (2013), 2; 245-255 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 641620 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
The effect of glucagon and cyclic adenosine monophosphate on acute liver damage induced by acetaminophen

Autori
Kelava, Tomislav ; Ćavar, Ivan ; Vukojević, Katarina ; Saraga-Babić, Mirna ; Ćulo, Filip

Izvornik
Histology and histopathology (0213-3911) 28 (2013), 2; 245-255

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
acute liver damage; acetaminophen; glucagon

Sažetak
Recent investigations suggest that glucagon might have a potentially important hepatoprotective activity. We investigated the effect of glucagon in a model of acetaminophen-induced liver injury. CBA male mice were injected intraperitoneally with a lethal (300 mg/kg) or sublethal (150 mg/kg) dose of acetaminophen. The liver injury was assessed by observing the survival of mice, by liver histology and by measuring the concentration of alanine-aminotransferase (ALT). Inducible nitric oxide synthase (iNOS) and nuclear factor kappa B (NF-κB) protein expressions were determined immunohistochemically. Hepatic levels of reduced glutathione (GSH) and cyclic adenosine monophosphate (cAMP) were also measured. Results show that glucagon, dose and time dependently, protects against acetaminophen-induced hepatotoxicity. This protection was achieved with a dose of 0.5 mg/kg of glucagon given intraperitoneally 15 min before or 1 h after acetaminophen. Treatment of animals with acetaminophen elevated ALT and nitrite/nitrate concentration in the plasma, enhanced iNOS and NF-κB expression and reduced GSH and cAMP concentration in the liver. Animals treated with glucagon had higher hepatic cAMP level, lower ALT and nitrite/nitrate concentration in plasma and lower expression of iNOS in liver cells than animals in control group, whereas there was no difference in the expression of NF-κB. Glucagon did not prevent the loss of GSH content caused by acetaminophen. Our investigation indicates that glucagon has a moderately protective effect against acetaminophen-induced liver injury, which is, at least partially, mediated through the downregulation of iNOS and through the increase in hepatic cAMP content, but it is not mediated through the modulation of NF-κB activity.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekti:
108-0000000-0328 - Uloga proupalnih citokina i prostaglandina u akutnom oštećenju jetre
108-1080229-0142 - Molekularni mehanizmi učinaka imunosnih poremećaja na kost (Grčević, Danka, MZOS ) ( POIROT)
216-2160528-0507 - Genski izražaj u ranom razvoju čovjeka (Saraga-Babić, Mirna, MZOS ) ( POIROT)
219-0000000-0328 - Uloga proupalnih citokina i prostaglandina u akutnom oštećenju jetre (Čulo, Filip, MZOS ) ( POIROT)

Ustanove:
Medicinski fakultet, Zagreb,
Medicinski fakultet, Split,
Medicinski fakultet, Osijek

Citiraj ovu publikaciju

Kelava, Tomislav; Ćavar, Ivan; Vukojević, Katarina; Saraga-Babić, Mirna; Ćulo, Filip
The effect of glucagon and cyclic adenosine monophosphate on acute liver damage induced by acetaminophen // Histology and histopathology, 28 (2013), 2; 245-255 (međunarodna recenzija, članak, znanstveni)
Kelava, T., Ćavar, I., Vukojević, K., Saraga-Babić, M. & Ćulo, F. (2013) The effect of glucagon and cyclic adenosine monophosphate on acute liver damage induced by acetaminophen. Histology and histopathology, 28 (2), 245-255.
@article{article, year = {2013}, pages = {245-255}, keywords = {acute liver damage, acetaminophen, glucagon}, journal = {Histology and histopathology}, volume = {28}, number = {2}, issn = {0213-3911}, title = {The effect of glucagon and cyclic adenosine monophosphate on acute liver damage induced by acetaminophen}, keyword = {acute liver damage, acetaminophen, glucagon} }
@article{article, year = {2013}, pages = {245-255}, keywords = {acute liver damage, acetaminophen, glucagon}, journal = {Histology and histopathology}, volume = {28}, number = {2}, issn = {0213-3911}, title = {The effect of glucagon and cyclic adenosine monophosphate on acute liver damage induced by acetaminophen}, keyword = {acute liver damage, acetaminophen, glucagon} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE





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