Impact of RNA interference with stemness gene- expression in experimental mouse teratoma grown in vitro (CROSBI ID 599588)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | domaća recenzija
Podaci o odgovornosti
Sinčić, Nino ; Gospodinov, Anastas ; Vlahović, Maja ; Jurić-Lekić, Gordana ; F. Bulić-Jakuš, Floriana
engleski
Impact of RNA interference with stemness gene- expression in experimental mouse teratoma grown in vitro
RNA interference provides an epigenetic mechanism that has been used in targeted silencing of gene expression at the mRNA level for therapy of human diseases (e.g. wet macular degeneration with bevasiranib- siRNA against VEGF). This investigation has been dedicated to discover impact on developmental parameters of the experimental mouse teratoma by silencing expression of stemness (Oct3/4, Nanog) and Trrap genes. E7, 5 C3H mouse embryos were isolated under the dissecting microscope and grown at the air-liquid interface in a serum-supplemented medium for seven days. Their axes were measured each day from the beginning of the 7-day-culture period by an ocular micrometer to assess overall growth of the tumor. esiRNAs were constructed according to following sequences: Oct3/4 (NM_013633.1), Nanog (XM_132755.3), Trrap (NM_133901.2), GFP (132-591) as a negative control. They were applied to the medium by lipofectamine 2000. In teratomas, derivatives of all three germ layers were discovered: epidermis, neural tissue, vegetative ganglionic cells, smooth muscle, myotubes, cylindrical epithelium and gastric epithelium with cells typical for the fundic region with parietal and chief cells. In comparison to esiGFP (control), esiOct3/4 and especially esiNanog have significantly diminished overall growth of teratomas. EsiTrrap did not significantly diminish overall growth, but it seems to have induced major apoptotic activity. Therefore it is possible that silencing of the stemness genes has had impact on the cell proliferation. On the other hand in esiTrapp treated teratomas, where stemness genes were not silenced, they probably could compensate for overall growth through cell proliferation despite of the seemingly pronounced apoptotic activity.
RNAi; mouse teratoma
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Podaci o prilogu
PP21-PP21.
2012.
objavljeno
Podaci o matičnoj publikaciji
Book of Abstracts
Zagreb:
Podaci o skupu
23rd Ljudevit Jurak International Symposium on Comparative Pathology
poster
01.06.2012-02.06.2012
Zagreb, Hrvatska