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Non-human errors are the main source of falsely determined alleles in DNA databases


Džijan, Snježana; Ćurić, Goran; Pavlinić, Dinko; Hercog, Rajna; Lauc, Gordan
Non-human errors are the main source of falsely determined alleles in DNA databases // The 6th ISABS Conference on Human Genome Project Based Applications in Forensic Science, Anthropology and Individualized Medicine: The book of abstracts / Vuk-Pavlović, Stanimir ; Primorac, Dragan ; Schanfield, Moses (ur.).
Zagreb: International Society for Applied Biological Sciences (ISABS), 2009. str. 79-79 (poster, međunarodna recenzija, sažetak, ostalo)


Naslov
Non-human errors are the main source of falsely determined alleles in DNA databases

Autori
Džijan, Snježana ; Ćurić, Goran ; Pavlinić, Dinko ; Hercog, Rajna ; Lauc, Gordan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
The 6th ISABS Conference on Human Genome Project Based Applications in Forensic Science, Anthropology and Individualized Medicine: The book of abstracts / Vuk-Pavlović, Stanimir ; Primorac, Dragan ; Schanfield, Moses - Zagreb : International Society for Applied Biological Sciences (ISABS), 2009, 79-79

Skup
The 6th ISABS Conference on Human Genome Project Based Applications in Forensic Science, Anthropology and Individualized Medicine

Mjesto i datum
Split, Hrvatska, 01-05.06.2009

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
DNA typing; Human identification; STR loci; DNA database; Quality control

Sažetak
Aiming to estimate frequency of various types of errors that may have occurred during the process of identification of victims of war in Croatia we compared genotypes of 911 parent-child pairs in the database of nearly 3500 relatives of missing people. By repeated analysis of mismatched genotypes we identified seven errors in the database. Out of these seven errors, falsely genotypes alleles were caused by human error, while the remaining five errors were caused by allelic dropout or electrophoretic aberrations. An important observation is that all three observed allelic dropouts occurred at D2S1338 locus which is the largest fragment amplified by the AmpFlSTR Identifiler kit. Larger alleles at this locus frequently amplify with lower efficiency, sometimes not reaching the detection level indicating that additional proofreading procedures should be performed when homozygous D2S1338 locus is being reported.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti



POVEZANOST RADA


Ustanove
Medicinski fakultet, Osijek

Profili:

Avatar Url Dinko Pavlinić (autor)

Avatar Url Gordan Lauc (autor)

Avatar Url Goran Ćurić (autor)

Avatar Url Snježana Džijan (autor)

Citiraj ovu publikaciju

Džijan, Snježana; Ćurić, Goran; Pavlinić, Dinko; Hercog, Rajna; Lauc, Gordan
Non-human errors are the main source of falsely determined alleles in DNA databases // The 6th ISABS Conference on Human Genome Project Based Applications in Forensic Science, Anthropology and Individualized Medicine: The book of abstracts / Vuk-Pavlović, Stanimir ; Primorac, Dragan ; Schanfield, Moses (ur.).
Zagreb: International Society for Applied Biological Sciences (ISABS), 2009. str. 79-79 (poster, međunarodna recenzija, sažetak, ostalo)
Džijan, S., Ćurić, G., Pavlinić, D., Hercog, R. & Lauc, G. (2009) Non-human errors are the main source of falsely determined alleles in DNA databases. U: Vuk-Pavlović, S., Primorac, D. & Schanfield, M. (ur.)The 6th ISABS Conference on Human Genome Project Based Applications in Forensic Science, Anthropology and Individualized Medicine: The book of abstracts.
@article{article, year = {2009}, pages = {79-79}, keywords = {DNA typing, Human identification, STR loci, DNA database, Quality control}, title = {Non-human errors are the main source of falsely determined alleles in DNA databases}, keyword = {DNA typing, Human identification, STR loci, DNA database, Quality control}, publisher = {International Society for Applied Biological Sciences (ISABS)}, publisherplace = {Split, Hrvatska} }