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Non-paternity and genetic inconsistencies in cases of disputed fatherhood in DNA laboratory in Osijek, Croatia

Uncertainty of paternity nowadays can be easily resolved with DNA testing. The method of DNA profiling used for determining whether two individuals have a biological parent–child relationship in Forensic DNA laboratories is based on PCR and the use of highly polymorphic short tandem repeats (STRs). The aim of this study is to establish (1) the rate of non-paternity in a sample from paternity testing laboratory, (2) association of non-paternity event with other available data and (3) type of inconsistencies between the obtained genotypes. The study is based on review of archive material from 248 cases of paternity testing during the 13 years (2001-2013) in Laboratory for DNA analysis in Medical school in Osijek, Croatia. Paternity testing was in 64.1% of cases ordered by the court (child as a plaintiff) and in 14.5% of cases requested privately by the father. Mothers` maiden name was found in 67.7% children, and in 12.5% of children had fathers` last name. Paternity testing was false in 13.3% of cases. Children with false paternity test were younger than children with positive paternity test (Me 0.41 years vs. Me 1.00 years ; p=0.049). Six mutations were observed within 3 480 allelic transfers at 15 STR-loci. Our non-paternity rate of 13.3% in population sample was among the lowest of published data from other paternity testing laboratories.

Non-paternity; fatherhood; paternity testing; forensics; STR loci

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