engleski

Effect of endemic plants extracts on biological macromolecules

Reactive oxygen species (ROS) are generated during normal aerobic metabolism, but they may be toxic in higher concentrations causing a great damage of macromolecules such as DNA, proteins and lipids. Oxidative stress which usually generates increased amount of ROS play an important role in an etiology and pathogenesis of various diseases such as cardiovascular disease, cancer, diabetes and neurodegenerative disease. Cells possess mechanisms for radical detoxification that are not full effective in case of drastic stress conditions. Some compounds with important antioxidant activity originated in food and dietary supplements may have protective effect on ROS caused damages. Thus, numerous epidemiological and clinical studies support hypothesis that regular consumption of products reach in antioxidants have protective effect in diseases mediated through deleterious free radical chain reactions. Medicinal plants which have been used in traditional medicine for many years are particularly in focus, although their biological activity and effects on biological macromolecules have not been investigated yet. Herein we investigated potential protecting effects of four endemic plants extracts (Teucrium arduini L., Moltkia petraea (Tratt.) Griseb, Micromeria croatica (Pers.) Schott and Rhamnus intermedia Steud. et Hochst) against ROS damaging of important biological macromolecules: lipids, proteins and DNA. Content of polyphenolic compounds (total phenol, flavonoids, hydroxycinnamic acids, proanthocyanidins and individual phenolic acids) was determined in plant extracts using spectrophotometer and UPLC-MS/MS. Furthermore, in vitro antioxidant activities of plant methanol extracts were screened using DPPH, ABTS and FRAP methods. All four tested extracts shown high in vitro antioxidant capacity and DNA protection effects. However, their effects on protein and lipid protection were diverse and will be discussed.

endemic plant; ROS; oxidative stress

nije evidentirano