QRT-PCR analysis of HMGA2 expression in FNA samples of thyroid papillary carcinoma and non-neoplastic thyroid lesions (CROSBI ID 598662)
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Podaci o odgovornosti
Mateša, Neven ; Šamija, Ivan ; Lukač, Josip ; Kusić, Zvonko
engleski
QRT-PCR analysis of HMGA2 expression in FNA samples of thyroid papillary carcinoma and non-neoplastic thyroid lesions
Objectives: To determine and compare HMGA2 expression in non-neoplastic thyroid lesions and thyroid papillary carcinoma in fine needle aspiration (FNA) samples by quantitative real-time RT-PCR (QRTPCR). Materials and Methods: QRT-PCR for HMGA2 expression was performed on RNA isolated from aspirates obtained by ultrasound guided FNA from 95 patients with papillary carcinoma, Hashimoto thyroiditis and nodular goitre. HMGA2 expression was quantified by DDCt method using pooled FNA samples from normal thyroid tissue as calibrator. Results of QRT-PCR analysis were evaluated against the definitive FNA diagnosis. Results:With a cut-off value of 11.69 determined by ROC curve analysis 18 out of 20 (90%) papillary carcinoma, 2 out of 28 (7%) Hashimoto thyroiditis and 6 out of 47 (13%) nodular goitre samples were positive for HMGA2 expression. HMGA2 expression has shown accuracy of 89.4%, sensitivity of 90.0% and specificity of 90.1% in discriminating between samples with papillary carcinoma and non-neoplastic thyroid lesions. Proportion of HMGA2 positive samples was significantly higher in samples with papillary carcinoma compared to samples with non-neoplastic lesions (p<0.0001, Fisher’s exact test). There was no significant difference in the proportion of positive samples between Hashimoto thyroiditis and nodular goitre samples. Conclusion: Analysis of HMGA2 expression by QRT-PCR could improve conventional thyroid cytology in diagnosis of papillary carcinoma
cytology; HMGA2; RT-PCR; thyroid
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Podaci o prilogu
32-32.
2013.
nije evidentirano
objavljeno
Podaci o matičnoj publikaciji
Acta cytologica
0001-5547
Podaci o skupu
18th International Congress of Cytology
predavanje
01.01.2013-01.01.2013
Pariz, Francuska
Povezanost rada
Temeljne medicinske znanosti, Kliničke medicinske znanosti