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Pregled bibliografske jedinice broj: 636469

Berberine exerts nephroprotective effect against cisplatin-induced kidney damage through inhibition of oxidative/nitrosative stress, inflammation, autophagy and apoptosis


Domitrović, Robert; Cvijanović, Olga; Pernjak- Pugel, Ester; Škoda, Marko; Mikelić, Lorena; Crnčević-Orlić, Željka
Berberine exerts nephroprotective effect against cisplatin-induced kidney damage through inhibition of oxidative/nitrosative stress, inflammation, autophagy and apoptosis // Food and chemical toxicology, 62 (2013), 397-406 doi:10.1016/j.fct.2013.09.003 (međunarodna recenzija, članak, znanstveni)


Naslov
Berberine exerts nephroprotective effect against cisplatin-induced kidney damage through inhibition of oxidative/nitrosative stress, inflammation, autophagy and apoptosis

Autori
Domitrović, Robert ; Cvijanović, Olga ; Pernjak- Pugel, Ester ; Škoda, Marko ; Mikelić, Lorena ; Crnčević-Orlić, Željka

Izvornik
Food and chemical toxicology (0278-6915) 62 (2013); 397-406

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Berberine; cisplatin nephrotoxicity; oxidative stress; inflammatory response; autophagy; apoptosis

Sažetak
The aim of this study was to investigate the therapeutic activity of isoquinoline alkaloid berberine against cisplatin (CP)-induced nephrotoxicity in mice. Berberine was administered at daily doses of 1, 2 and 3 mg/kg by gavage for two succesive days, 48 h after intraperitoneal CP injection (13 mg/kg). Mice were sacrificed 24 h after the last dose of berberine. Histopathological changes and the increase in serum creatinine and blood urea nitrogen (BUN) induced by CP were significantly ameliorated by berberine in a dose-dependent manner. Additionally, oxidative/nitrosative stress, evidenced by the increase in renal 4- hydroxynonenal (4-HNE), 3-nitrotyrosine (3-NT), cytochrome P450 E1 (CYP2E1) and heme oxygenase (HO-1) expression, was significantly reduced. The expression of nuclear factor-kappaB (NF- κB), tumor necrosis factor-α (TNF-α), cyclooxygenase-2 (COX- 2) and inducible nitric oxide synthase (iNOS) was markedly suppressed by berberine, indicating the inhibition of inflammatory response. Treatment of CP- intoxicated animals with berberine also significantly reduced the expression of p53, active caspase-3 as well as autophagy marker light chain 3B (LC3B) in the kidneys. The results of the current study showed the nephroprotective activity of berberine against CP-induced renal injury, which could be attributed to the inhibition of oxidative/nitrosative stress, inflammation, autophagy and apoptosis.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Farmacija



POVEZANOST RADA


Projekt / tema
062-0000000-3554 - Aktivni sastojci ljekovitog bilja u terapiji fibroze jetre (Robert Domitrović, )
062-0620226-0208 - Kost kao ciljni organ u šećernoj bolesti i upalnim bolestima crijeva (Željka Crnčević Orlić, )
062-0621261-1269 - Perinatalni citomegalovirusni encefalitis (Ester Pernjak-Pugel, )
potpora 13.06.1.2.24
Sveučilište u Rijeci

Ustanove
Medicinski fakultet, Rijeka,
Klinički bolnički centar Rijeka

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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