engleski

Influence of organic selenium feed supplement and fasting on oxidative damage in different tissues of broiler chickens

The influence of organic selenium feed supplements on lipid peroxidation and protein oxidation in different tissues of Ross 308 broiler chickens of both sexes in response to 48 hours food deprivation were investigated. Chickens were randomly allocated into standard diet fed control group (N=50) and experimental group, pair-fed with the same diet supplemented with 0.3 ppm organic selenium (Sel-Plex®, Alltech, Inc., KY ; N=50). At the age of 42 days and after 48 hours food deprivation ten chickens of experimental group and ten chickens of control were sacrificed and liver, kidney and small intestine were removed. Liver, small intestine and kidney tissue homogenates were analysed for lipid peroxide concentrations (LPO) and protein carbonyl content (PCC). The PCC in kidney and small intestine of experimental chickens at the end of fattening period was higher than in control broilers (P<0.05). As a result of the food deprivation, lower PCC was found in liver of both groups (P<0.05). Simultaneously, the values for experimental group were lower than in the control group (P<0.05). Kidney PCC was increased after fasting only in control birds (P<0.05). The LPO in kidney at the end of fattening was higher in chickens fed diet supplemented with organic selenium (P<0.05). Kidney and small intestine tissue LPO of both groups were decreased after fasting (P<0.05). At the same time LPO in liver of experimental group was increased (P<0.05). Results of this study demonstrated that dietary organic selenium supplements increased oxidative damage in kidney and small intestine at the end of fattening. Food deprivation resulted in decreased tissue oxidative damage due to lower metabolic rate.

chicken; organic selenium; food deprivation; lipid peroxidation; protein oxidation

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