engleski

Glucosinolates, volatile constituents, and acetylcholinesterase inhibitory activity of Alyssoides utriculata

Glucosinolates (GLs) and volatiles from all plant parts of Alyssoides utriculata (L.) Medik were determined for the first time. Total GLs determined by ferricyanide assay were 45.7, 12.2, 7.3, 12.5 and 15.7 μmol/g dry weight in the seed, root, stem, leaf and flower, respectively. Qualitative determination of individual GLs was done indirectly by GC/MS analysis of volatiles isolated by hydrodistillation, as well as by CH2Cl2 extraction after enzyme hydrolysis. The extracts were shown to contain mostly volatiles originating from degradation of gluconapin, glucoerucin, and glucoraphanin. The volatile isolates, tested for their acetylcholinesterase inhibition according to Ellman method, were dose-dependent reaching 53.9% and 53.3% at the final concentration of 320 μg/mL, for seed CH2Cl2 extract and hydrodistillate of the whole plant, respectively.

Alyssoides utriculata; glucosinolates; acetylcholinesterase inhibition; GC/MS

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