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Kinetic Spectrophotometric Determination of N-acetyl-L-cysteine based on the reduction of copper(II)-neocuproine reagent (CROSBI ID 192861)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Radić, Njegomir ; Kukoč Modun, Lea ; Biočić, Maja Kinetic Spectrophotometric Determination of N-acetyl-L-cysteine based on the reduction of copper(II)-neocuproine reagent // Croatica chemica acta, 86 (2013), 1; 65-71. doi: 10.5562/cca2161

Podaci o odgovornosti

Radić, Njegomir ; Kukoč Modun, Lea ; Biočić, Maja

engleski

Kinetic Spectrophotometric Determination of N-acetyl-L-cysteine based on the reduction of copper(II)-neocuproine reagent

Novel simple kinetic spectrophotometric method for the determination of N-acetyl-L-cysteine (NAC) has been developed and validated. The proposed method is based on the reduction of CuII-neocuproine reagent to CuI-neocuproine with the analyte, in a Britton-Robinson buffer solution (pH = 3.0). The non-steady state absorbance of the formed CuI-neocuproine complex is measured at 458 nm. The initial rate and fixed time (at 1 min) methods were utilized for constructing the calibration graphs. The graphs for both methods were linear in concentration range from 6.0  107 to 8.0  105 mol L1 with the limit of detection 1.7  107 mol L1. The slope of the initial method calibration curve (1.0181) confirmed the first order reaction. The both proposed methods were successfully applied for the determination of NAC in its commercial pharmaceutical formulations.

N-acetyl-L-cysteine; Kinetic spectrophotometry; Initial rate method; Fixed time method; Pharmaceutical analysis

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Podaci o izdanju

86 (1)

2013.

65-71

objavljeno

0011-1643

10.5562/cca2161

Povezanost rada

Kemija

Poveznice
Indeksiranost