Kinetic Spectrophotometric Determination of N-acetyl-L-cysteine based on the reduction of copper(II)-neocuproine reagent (CROSBI ID 192861)
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Radić, Njegomir ; Kukoč Modun, Lea ; Biočić, Maja
engleski
Kinetic Spectrophotometric Determination of N-acetyl-L-cysteine based on the reduction of copper(II)-neocuproine reagent
Novel simple kinetic spectrophotometric method for the determination of N-acetyl-L-cysteine (NAC) has been developed and validated. The proposed method is based on the reduction of CuII-neocuproine reagent to CuI-neocuproine with the analyte, in a Britton-Robinson buffer solution (pH = 3.0). The non-steady state absorbance of the formed CuI-neocuproine complex is measured at 458 nm. The initial rate and fixed time (at 1 min) methods were utilized for constructing the calibration graphs. The graphs for both methods were linear in concentration range from 6.0 107 to 8.0 105 mol L1 with the limit of detection 1.7 107 mol L1. The slope of the initial method calibration curve (1.0181) confirmed the first order reaction. The both proposed methods were successfully applied for the determination of NAC in its commercial pharmaceutical formulations.
N-acetyl-L-cysteine; Kinetic spectrophotometry; Initial rate method; Fixed time method; Pharmaceutical analysis
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