engleski

Loss of heterozygosity of selected tumor suppressor genes in human testicular germ cell tumors

Human testicular germ cell tumors (TGCT) are histologically heterogenous group of neoplasms with variable malignant potential. Two main types of germ cell tumors occur in men: seminomas and nonseminomas, later generally having poorer prognosis. In the present study a set of tumor suppressor genes (CDH1, APC, p53 and nm23-H1) was investigated in testicular cancer for loss of heterozygosity (LOH). Both seminoma and nonseminoma tissue samples were analysed by PCR using either restriction fragment length polymorphism or dinucleotide or tetranucleotide repeat polymorphism method. The results of our analysis showed that the most frequent change was LOH of p53, although allelic losses of APC and CDH1 were also observed. No consistent LOH has been observed in all of the tumor types, and overall incidence of LOH of tumor suppressor genes in TGCT has shown to be about 15%.

loss of heterozygosity; tumor suppressors; testicular germ cell tumors

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