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Pregled bibliografske jedinice broj: 626498

Detection of Bovine viral diarrhea virus-specific neutralizing antibodies in fresh colostrum : A modification of the virus neutralization test


Bedeković, Tomislav; Mihaljević, Željko; Jungić, Andreja; Lemo, Nina; Lojkić, Ivana; Cvetnić, Željko; Čač, Željko
Detection of Bovine viral diarrhea virus-specific neutralizing antibodies in fresh colostrum : A modification of the virus neutralization test // Journal of veterinary diagnostic investigation, 25 (2013), 2; 263-266 doi:10.1177/1040638713478813 (međunarodna recenzija, članak, znanstveni)


Naslov
Detection of Bovine viral diarrhea virus-specific neutralizing antibodies in fresh colostrum : A modification of the virus neutralization test

Autori
Bedeković, Tomislav ; Mihaljević, Željko ; Jungić, Andreja ; Lemo, Nina ; Lojkić, Ivana ; Cvetnić, Željko ; Čač, Željko

Izvornik
Journal of veterinary diagnostic investigation (1040-6387) 25 (2013), 2; 263-266

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Bovine viral diarrhea virus; fresh colostrum; specific antibodies; virus neutralization test

Sažetak
To eliminate cytotoxic effects of colostrum on cells, a modified virus neutralization test (VNT) for the detection of Bovine viral diarrhea virus- specific neutralizing antibodies in colostrum was developed. The new test was compared to the World Organization for Animal Health-recommended VNT and the results evaluated. The agreement of the new test compared to the standard VNT was determined to be 98%, whereas sensitivity and specificity of the modified VNT compared to the standard VNT were 100%. Bovine viral diarrhea virus-specific antibodies were detected in 42 sera samples and 38 colostrum samples. The antibody titers in serum and colostrum showed a high correlation (n = 56, r = 0.9719, P < 0.001). The modified virus neutralization technique described herein succeeds in eliminating cytotoxic effects and can be readily applied for the detection of specific antibodies against other infectious agents in colostrum.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina



POVEZANOST RADA


Projekt / tema
048-0481153-1127 - Istraživanje bolesti životinja s neurološkim poremećajima (Branko Šoštarić, )
048-0481153-1150 - Molekularna epizootiologija važnih bakterijskih zoonoza (Željko Cvetnić, )
048-0481186-1183 - Primjena genetičkih analiza u veterinarskoj medicini (Mirko Lojkić, )

Ustanove
Hrvatski veterinarski institut, Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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