engleski

Paraoxonase genotype distribution in cardiovascular dieseases

Paraoxonase (PON1) genetic polymorphism at codon 192 (glutamine to arginine substitution, genotypes A and B, respectively) has been shown to affect the risk of CVD. The aim of this study was to genotype 44 controls and 100 CVD patients with different diagnosis (coronary artery disease (CAD), myocardial infarction (MI), hypertension and carotid stenosis (CS)) by PCR-RFLP using Alw1 restriction enzyme. Study results showed that out of 44 controls 18/44 (40.9%) were A and 1/44 (2.3%) B homozygotes and 25/44 (56.8%) heterozygotes. Among 31 CAD patients 9/31 (29.0%) were A and 5/31 (16.1%) B homozygotes and 17/31 (54.8%) heterozygotes. In 27 MI patients 8/27 (29.6%) were A and 4/27 (14.8%) B homozygotes and 15/27 (55.6%) heterozygotes. Out of 16 hypertension patients 6/16 (37.5%) were A and 3/16 (18.8%) B homozygotes and 7/16 (43.8%) heterozygotes. Among 26 CS patients 15/26 (57.7%) were A and 2/26 (7.7%) B homozygotes and 9/26 (34.6%) heterozygotes. Genotypes between controls and patients differed significantly (Chi-square test; P=0.002, 0.003, <0.001 and 0.003; for CAD, MI, Hypertension and CS patients, respectively). Allelic frequencies did not differ significantly between controls and patients. Our findings indicate the association between the codon 192 polymorphism of PON1 gene and cardiovascular diseases.

paraoxonase; CVD

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