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Pregled bibliografske jedinice broj: 625156

Identification of P-Glycoprotein Inhibitors in Contaminated Freshwater Sediments


Zaja, Roko; Terzić, Senka; Senta, Ivan; Lončar, Jovica; Popović, Marta; Ahel, Marijan; Smital, Tvrtko
Identification of P-Glycoprotein Inhibitors in Contaminated Freshwater Sediments // Environmental science & technology, 47 (2013), 9; 4813-4821 doi:10.1021/es400334t (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 625156 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Identification of P-Glycoprotein Inhibitors in Contaminated Freshwater Sediments

Autori
Zaja, Roko ; Terzić, Senka ; Senta, Ivan ; Lončar, Jovica ; Popović, Marta ; Ahel, Marijan ; Smital, Tvrtko

Izvornik
Environmental science & technology (0013-936X) 47 (2013), 9; 4813-4821

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
multixenobiotic resistance ; P-glycoprotein inhibitors ; contaminated sediments ; effect-directed analysis (EDA) ; nonionic surfactants

Sažetak
P-glycoprotein (P-gp, ABCB1) is an important part of the multixenobiotic resistance (MXR) defense system in aquatic organisms. The main goal of this study was identification of P-gp inhibitors in contaminated sediments using the effect-directed analysis (EDA) approach. The samples were collected from the Gorjak creek (Zagreb, Croatia), a recipient of wastewater effluents from the pharmaceutical industry. Sediment samples were extracted and fractionated using two-tiered approach. Resulting non-polar, medium polar and polar fractions were tested on the inhibition of P-gp activity using P-gp overexpressing PLHC-1/dox cells and calcein-AM as model substrate. The obtained EC50 values - up to 757 μg/g, expressed in toxicity equivalents of model P-gp inhibitor cyclosporine A - revealed high inhibitory potential of polar fractions of investigated sediments and clearly reflected the impact of pharmaceutical wastewater. P-gp specific ATPase assay and the cytotoxicity modulation experiments with colchicine indicated that most of the observed P-gp inhibition was due to the presence of non-competitive inhibitors. A detailed chemical analysis by ultrahigh-performance liquid chromatography-quadrupole/time-of-flight mass spectrometry (UPLC-QToFMS) revealed nonionic surfactants, including alcohol polyethoxylates (LAEOs) and polypropylene glycols (PPGs), as the major components of the most active sub-fractions. Testing of several LAEO and PPG commercial mixtures confirmed their potential to inhibit the fish P-glycoprotein and modulate toxicity of other xenobiotics present in complex environmental samples.

Izvorni jezik
Engleski

Znanstvena područja
Geologija, Biologija



POVEZANOST RADA


Projekt / tema
098-0982934-2712 - Organski spojevi kao molekulski obilježivači antropogenog utjecaja na okoliš (Marijan Ahel, MZOS)
098-0982934-2745 - Ekotoksikološko značenje ABC transportnih proteina u vodenih organizama (Tvrtko Smital, MZOS)

Ustanove
Institut "Ruđer Bošković", Zagreb

Profili:

Avatar Url Marta Popović (autor)

Avatar Url Tvrtko Smital (autor)

Avatar Url Marijan Ahel (autor)

Avatar Url Ivan Senta (autor)

Avatar Url Senka Terzić (autor)

Avatar Url Roko Žaja (autor)

Avatar Url Jovica Lončar (autor)

Citiraj ovu publikaciju

Zaja, Roko; Terzić, Senka; Senta, Ivan; Lončar, Jovica; Popović, Marta; Ahel, Marijan; Smital, Tvrtko
Identification of P-Glycoprotein Inhibitors in Contaminated Freshwater Sediments // Environmental science & technology, 47 (2013), 9; 4813-4821 doi:10.1021/es400334t (međunarodna recenzija, članak, znanstveni)
Zaja, R., Terzić, S., Senta, I., Lončar, J., Popović, M., Ahel, M. & Smital, T. (2013) Identification of P-Glycoprotein Inhibitors in Contaminated Freshwater Sediments. Environmental science & technology, 47 (9), 4813-4821 doi:10.1021/es400334t.
@article{article, year = {2013}, pages = {4813-4821}, DOI = {10.1021/es400334t}, keywords = {multixenobiotic resistance, P-glycoprotein inhibitors, contaminated sediments, effect-directed analysis (EDA), nonionic surfactants}, journal = {Environmental science and technology}, doi = {10.1021/es400334t}, volume = {47}, number = {9}, issn = {0013-936X}, title = {Identification of P-Glycoprotein Inhibitors in Contaminated Freshwater Sediments}, keyword = {multixenobiotic resistance, P-glycoprotein inhibitors, contaminated sediments, effect-directed analysis (EDA), nonionic surfactants} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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