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Pregled bibliografske jedinice broj: 624366

Adaptation of Aminoacyl-tRNA Synthetase Catalytic Core to Carrier Protein Aminoacylation


Močibob, Marko; Ivić, Nives; Luić, Marija; Weygand- Đurašević, Ivana
Adaptation of Aminoacyl-tRNA Synthetase Catalytic Core to Carrier Protein Aminoacylation // Structure, 21 (2013), 4; 614-626 doi:10.1016/j.str.2013.02.017 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 624366 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Adaptation of Aminoacyl-tRNA Synthetase Catalytic Core to Carrier Protein Aminoacylation

Autori
Močibob, Marko ; Ivić, Nives ; Luić, Marija ; Weygand- Đurašević, Ivana

Izvornik
Structure (0969-2126) 21 (2013), 4; 614-626

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
aaRS homologs ; amino acid:[carrier protein] ligase ; carrier protein ; noncanonical functions of aaRS ; seryl-tRNA synthetase

Sažetak
Amino acid:[carrier protein] ligases (aa:CP ligases) are newly discovered enzymes that are highly similar to class II aminoacyl-tRNA synthetases (aaRSs). However, while aaRSs aminoacylate tRNA and supply building blocks for ribosomal translation, aa:CP ligases transfer activated amino acids to the phosphopantetheine group of small carrier proteins. We have solved the crystal structure of an aa:CP ligase complexed with the carrier protein (CP). The CP prosthetic group enters the active site from a different direction than tRNA in class II aaRS complexes, through an idiosyncratic tunnel. CP binds to aa:CP ligase in fundamentally different manner compared to tRNA binding by structurally closely related aaRSs. Based on crystallographic analysis, an enzyme of altered CP specificity was designed, and the mechanism of amino acid transfer to the prosthetic group was proposed. The presented study reveals how conserved class II aaRS catalytic core can adapt to a novel function through minor structural alterations.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija



POVEZANOST RADA


Projekti:
HRZZ-IS-09.01/293 - Nekanonske uloge aminoacil-tRNA-sintetaza
098-1191344-2943 - Protein-ligand međudjelovanja na atomnoj razini (Luić, Marija, MZOS ) ( POIROT)
119-0982913-1358 - Strukturna raznolikost seril-tRNA sintetaza i točnost biosinteze proteina (Rokov Plavec, Jasmina; Weygand Đurašević, Ivana, MZOS ) ( POIROT)

Ustanove:
Institut "Ruđer Bošković", Zagreb,
Prirodoslovno-matematički fakultet, Zagreb

Poveznice na cjeloviti tekst rada:

doi www.cell.com www.sciencedirect.com

Citiraj ovu publikaciju:

Močibob, Marko; Ivić, Nives; Luić, Marija; Weygand- Đurašević, Ivana
Adaptation of Aminoacyl-tRNA Synthetase Catalytic Core to Carrier Protein Aminoacylation // Structure, 21 (2013), 4; 614-626 doi:10.1016/j.str.2013.02.017 (međunarodna recenzija, članak, znanstveni)
Močibob, M., Ivić, N., Luić, M. & Weygand- Đurašević, I. (2013) Adaptation of Aminoacyl-tRNA Synthetase Catalytic Core to Carrier Protein Aminoacylation. Structure, 21 (4), 614-626 doi:10.1016/j.str.2013.02.017.
@article{article, author = {Mo\v{c}ibob, Marko and Ivi\'{c}, Nives and Lui\'{c}, Marija and Weygand- \DJura\v{s}evi\'{c}, Ivana}, year = {2013}, pages = {614-626}, DOI = {10.1016/j.str.2013.02.017}, keywords = {aaRS homologs, amino acid:[carrier protein] ligase, carrier protein, noncanonical functions of aaRS, seryl-tRNA synthetase}, journal = {Structure}, doi = {10.1016/j.str.2013.02.017}, volume = {21}, number = {4}, issn = {0969-2126}, title = {Adaptation of Aminoacyl-tRNA Synthetase Catalytic Core to Carrier Protein Aminoacylation}, keyword = {aaRS homologs, amino acid:[carrier protein] ligase, carrier protein, noncanonical functions of aaRS, seryl-tRNA synthetase} }
@article{article, author = {Mo\v{c}ibob, Marko and Ivi\'{c}, Nives and Lui\'{c}, Marija and Weygand- \DJura\v{s}evi\'{c}, Ivana}, year = {2013}, pages = {614-626}, DOI = {10.1016/j.str.2013.02.017}, keywords = {aaRS homologs, amino acid:[carrier protein] ligase, carrier protein, noncanonical functions of aaRS, seryl-tRNA synthetase}, journal = {Structure}, doi = {10.1016/j.str.2013.02.017}, volume = {21}, number = {4}, issn = {0969-2126}, title = {Adaptation of Aminoacyl-tRNA Synthetase Catalytic Core to Carrier Protein Aminoacylation}, keyword = {aaRS homologs, amino acid:[carrier protein] ligase, carrier protein, noncanonical functions of aaRS, seryl-tRNA synthetase} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Citati:





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