engleski

Comparative SSR polymorphism analysis of winter and spring barley germplasm

In this research we used specific SSR markers (Bmac209, GMS21 and Bmac67) on a set of 126 barley cultivars (78 winter type and 48 spring type). Markers mentioned here have been previously confirmed through a number of publications as markers which point to certain chromosomal regions responsible for trait of high grain yield. Overall approach was to methodologically combine general variability survey with group testing of cultivars to a specific trait. Expectations were focused on finding an analogy between formed groups (genotype wise) and field results considering the same trait (grain yield). Two years (2010-2012) of field trials on the location of Osijek were set up in order to observe grain yield variations among cultivars. Results of molecular analysis show UPGMA dendrograms with clear distinction considering general variability (winter vs. spring varieties) and also within the major group (sub-grouping with reference to observed trait). A total of six sub-groups were detected among winter and four among spring types. High yielding groups among the chosen subset of relevant barley varieties in 9 winter and 9 spring types are: winter t. – group I and V, spring t. – group I. All of the calculated dendrograms are congruent to grain yield means of a chosen subset which confirms the overall marker ability to detect high yielding genotypes which can be used as a pre-breeding method in progeny check-up.

molecular markers; UPGMA; yield; grouping

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