Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 621969

Improving the Selectivity of Antimicrobial Peptides from Anuran Skin


Kamech, Nédia; Vukičević, Damir; Ladram, Ali; Piesse, Christophe; Vasseur, Julie; Bojović, Viktor; Simunić, Juraj; Juretić, Davor
Improving the Selectivity of Antimicrobial Peptides from Anuran Skin // Journal of chemical information and modeling, 52 (2012), 12; 3341-3351 doi:10.1021/ci300328y (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 621969 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Improving the Selectivity of Antimicrobial Peptides from Anuran Skin

Autori
Kamech, Nédia ; Vukičević, Damir ; Ladram, Ali ; Piesse, Christophe ; Vasseur, Julie ; Bojović, Viktor ; Simunić, Juraj ; Juretić, Davor

Izvornik
Journal of chemical information and modeling (1549-9596) 52 (2012), 12; 3341-3351

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
antimicrobial peptides; peptide software; drug design; anura; ascaphin-8; XT-7

Sažetak
Anuran skin is known to be a rich source of antimicrobial peptides although their therapeutic potential is often limited due to their toxicity against mammalian cells. The analysis of structure −activity relationships among anuran antimicrobial peptides provided the parameters to construct the “Mutator” tool for improving their selectivity for bacterial cells, by suggesting appropriate point substitutions. Double substitution analogues [K2, K16] of the Xenopus tropicalis peptide XT-7 and [I2, K19] of the Ascaphus truei peptide ascaphin-8 were predicted by this tool to have an increased ‘therapeutic index’ (TI = HC50/MIC for erythrocytes with respect to bacteria) > 80. The mutated peptides were synthesized and respectively found to have experimental TI values > 130 for S. aureus or E. coli, a considerable improvement with respect to TI < 37 for the parent compounds. Circular dichroism studies of the mutated peptides suggested this may in part be due to variations in the α-helical structure. For P. aeruginosa, which is more resistant to XT-7, the TI increased in the mutated peptide from 5 to >270, also due to a significant improvement in minimal inhibitory concentration. We have shown that the Mutator tool is capable of suggesting limited variations in natural anuran peptides capable of increasing peptide selectivity, by decreasing toxicity against mammalian erythrocytes, in general without compromising antibacterial activity. The tool is freely available on the Mutator Web server at http://split4.pmfst.hr/mutator/.

Izvorni jezik
Engleski

Znanstvena područja
Fizika, Kemija, Biologija



POVEZANOST RADA


Projekt / tema
177-1770495-0476 - Razvoj i primjene principa maksimalne proizvodnje entropije (Davor Juretić, )

Ustanove
Prirodoslovno-matematički fakultet, Split

Citiraj ovu publikaciju

Kamech, Nédia; Vukičević, Damir; Ladram, Ali; Piesse, Christophe; Vasseur, Julie; Bojović, Viktor; Simunić, Juraj; Juretić, Davor
Improving the Selectivity of Antimicrobial Peptides from Anuran Skin // Journal of chemical information and modeling, 52 (2012), 12; 3341-3351 doi:10.1021/ci300328y (međunarodna recenzija, članak, znanstveni)
Kamech, N., Vukičević, D., Ladram, A., Piesse, C., Vasseur, J., Bojović, V., Simunić, J. & Juretić, D. (2012) Improving the Selectivity of Antimicrobial Peptides from Anuran Skin. Journal of chemical information and modeling, 52 (12), 3341-3351 doi:10.1021/ci300328y.
@article{article, year = {2012}, pages = {3341-3351}, DOI = {10.1021/ci300328y}, keywords = {antimicrobial peptides, peptide software, drug design, anura, ascaphin-8, XT-7}, journal = {Journal of chemical information and modeling}, doi = {10.1021/ci300328y}, volume = {52}, number = {12}, issn = {1549-9596}, title = {Improving the Selectivity of Antimicrobial Peptides from Anuran Skin}, keyword = {antimicrobial peptides, peptide software, drug design, anura, ascaphin-8, XT-7} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Citati





    Contrast
    Increase Font
    Decrease Font
    Dyslexic Font