engleski

Serum paraoxonase activities in endemic nephropathy patients on long-term hemodialysis.

The aim of this study is to determine whether paraoxonase activity, paraoxonase phenotype and lipid status are altered in endemic nephropathy (EN) patients on long-term hemodialysis treatment as compared to healthy population. EN patients (n=31) and control subjects (n=145) were residents of the area of Slavonski Brod, Croatia. Paraoxon was used as a substrate for measuring basal and sodium chloride-stimulated (NaCl-stimulated) paraoxonase activity, and phenylacetate for measuring arylesterase activity. The double substrate method was used to assign phenotypes. Cholesterol, triglycerides, and high-density lipoprotein cholesterol (HDL-cholesterol) were determined by methods routinely used in medical- biochemical laboratories. Results are expressed as median values and tested by Mann-Whitney rank sum test. The values p<0.05 were considered significant. Basal and NaCl-stimulated paraoxonase activity as well as arylesterase activity were significantly lower in the patient group compared to controls ; 68% (p<0.001), 71% (p<0.001), and 58% (p<0.001), respectively. The distribution of paraoxonase phenotypes in the patient group and controls was as follows: AA, 45% and 39% ; AB, 37% and 48% ; BB 185 and 13%, respectively. Cholesterol and HDL-cholesterol were significantly decreased (87%, p<0.001 and 71% p<0.001, respectively), whereas triglycerides were significantly increased (195 %, p<0.001) in the patient group compared to control median value. We conclude that endemic nephropathy patients on long-term hemodialysis have decreased paraoxonase /arylesterase activity, which may indicate a greater risk of premature atherogenesis.

Endemic nephropathy; hemodialysis

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