How do endosymbiotic algae from green hydra respond to hydra growing media? (CROSBI ID 594806)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | domaća recenzija
Podaci o odgovornosti
Kovačević, Goran ; Čolić, Ivan ; Cvrtila, Adam ; Ivšić, Martina
engleski
How do endosymbiotic algae from green hydra respond to hydra growing media?
In this research we studied how different growth media for hydras affect endosimbiotic algae present in green hydra (Hydra viridissima Pallas, 1766) (Figure 1). We used three standard solutions for hydra growth prepared in aerated water: CN (NaHCO3, CaCl2), KNC (NaHCO3, KCl, CaCl2) and M (tris-HCl, NaHCO3, KCl, MgCl2, CaCl2) [1]. Dilution of all the solutions was 1/40. We placed 10 hydras in each of the solutions, one group in the glass-window refrigerator and the other at the room temperature. The experiment lasted for five days after which period algae were extracted by maceration (Figure 2). By light microscope Nikon Eclipse E600 we performed morphological observations: division, shape, color and cloroplasts of the algal cells. Micrographs were made by digital camera Nikon DXM1200. In CN and KNC solutions cell divisons to up to three daughter cells were observed and also a wide range of cell sizes. Chlorplasts were of regular, cup-shaped form. Algae were regularly round-shaped and green. In M solution the most successful result in terms of cell division where cells divided to up to four daughter cells was noticed. Chloroplasts seemed to have some irregularities in the apperance (dot-like inclusions). Cells seem to appear green-yellow and were regular in shape (Figures 3 and 4). It seems that the room teperature in general showed better conditions for endosymbiotic agal growth. Based on the results obtained, we concluded that endosymbiotic algae managed to grow best in the M solution, although cells seemed to change the color. In the end all the three culture solutions showed benefit for algal growth and maintenance of hydra. For comparison, the best solution for growing green hydra was KNC solution (1:20) where budding was recorded. After these preliminary obeservations more detailed light microscopy including morphometry as well as electron microscopy analysis should be performed to reveal more details.
hydra; endosymbiotic alga; growing solutions; morphology
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Podaci o prilogu
61-62.
2012.
objavljeno
Podaci o matičnoj publikaciji
Croatian Microscopy Symposium/Hrvatski mikroskopijski simpozij Book of Abstracts/Knjiga sažetaka
Gajović, Andreja ; Tomašić, Nenad
Zagreb: Hrvatsko mikroskopijsko društvo
978-953-57138-1-4
Podaci o skupu
Croatian Microscopy Symposium/Hrvatski mikroskopijski simpozij
poster
16.11.2012-17.11.2012
Pula, Hrvatska