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An electrochemical method for functionalization of a 316L stainless steel surface being used as a stent in coronary surgery : irreversible immobilization of fibronectin for the enhancement of endothelial cell attachment (CROSBI ID 191009)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Harvey, Jeffrey ; Bergdahl, Andreas ; Dadafarin, Hesam ; Ling, Li ; Davies, Elaine ; Omanović, Saša An electrochemical method for functionalization of a 316L stainless steel surface being used as a stent in coronary surgery : irreversible immobilization of fibronectin for the enhancement of endothelial cell attachment // Biotechnology letters, 34 (2012), 6; 1159-1165. doi: 10.1007/s10529-012-0885-8

Podaci o odgovornosti

Harvey, Jeffrey ; Bergdahl, Andreas ; Dadafarin, Hesam ; Ling, Li ; Davies, Elaine ; Omanović, Saša

engleski

An electrochemical method for functionalization of a 316L stainless steel surface being used as a stent in coronary surgery : irreversible immobilization of fibronectin for the enhancement of endothelial cell attachment

An electrochemistry-based method for the formation of functionalized alkanethiol layers on a 316L stainless steel surface was developed. The method was found to be efficient in forming a very stable, irreversibly-attached COOH-terminated (mercaptoundecanoic acid) surface layer. This layer was used as a ‘linker’ to immobilize the extracellular matrix protein fibronectin to the 316L stainless steel surface. It was shown that this fibronectin is irreversibly attached to the surface, and unlike physisorbed fibronectin, resists more to detachment in aggressive 0.1 M NaOH under sonication. The fibronectin-modified 316L stainless steel surface was shown to be more biocompatible towards attachment of endothelial cells than a bare (unmodified) 316L stainless steel surface, yielding a 25% improvement in cell density.

alkanetihol layers; cell/surface interactions; fibronection; stainless steel; surface functionalization

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Podaci o izdanju

34 (6)

2012.

1159-1165

objavljeno

0141-5492

10.1007/s10529-012-0885-8

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Kemija

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