engleski

Effects of Dopaminergic Drugs on Inflammatory Bowel Disease Induced with 2,4-dinitrofluorobenzene in BALB/c Mice

The aim of this study was to demonstrate the effects of dopaminergic drugs on experimental Inflammatory Bowel Disease (IBD) induced with 2,4-dinitroflurbenzene (DNFB) in previously sensitized BALB/c mice. Material and Methods. The 180 BALB/c mice were divided into three groups of 60 animals each. The mice in the first experimental group were treated with domperidone, a peripheral dopamine antagonist. The mice from the second experimental group were treated with bromcriptine, dopamine agonist. The mice from control group were treated with an equivolume of normal saline in the same manner. Ten animal from each group sere sacrificed on days 1, 2, 3, 5, 10, 20 subsequent to the challange enema of DNFB solution. Gross and microscopic examination of the colon were performed. The number and extent of ulcerations and erosions, the intensity of hemorrhages, oedema, presence of neutrophils and eosinophils, intensity and dynamics of mononuclear cell accumulation within colonic lamina propria and submucosa and giant cells granulomas were assessed. Results. In the applied animal model of IBD bromcriptine induced clinical improvement and decreased mortality rate 2/60 (3%), while domperidone effect was opposite and mortality rate was 12/60 (20%). Conclusion. The analysis of the total score of colonic lesions in animals from the experimental and the control groups showed protective effects of dopamine agonist bromcriptine on gut mucosa, while dopamine antagonist domperidone aggravated colonic lesions.

inflammatory bowel disease; dopaminergic drugs; 2;4-DNFB; BALB/c mice

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