engleski

Assessment of primary DNA damage on PK15 cell line after 24 and 48 hours exposure to silver nanoparticles in vitro

The need for nanotoxicity assesssment is becoming increasingly uppermost due to the increasing production of nanomaterials. Pk15 cells were used to study primary DNA damage after the exposure to six different concentrations of citrate-coated silver nanoparticles (AgNPs) after 24 and 48 hours treatment. . Exposure to 1 μg/mL of AgNO3 was done for comparison. Primary DNA damage was measured by the alkaline comet assay. Tail length, tail intensity and tail moment were measured. All experiments were performed in triplicate. In total, 300 comets per concentration were measured. Stable and purified AgNPs were prepared in sterile ultrapure water. Results showed that genotoxic activity of the AgNPs was both concentration and time dependent. Control experiments with ultrapure water showed effects on cell number and cell damage, but these effects seemed to be beneficial when compared with untreated cells. Thus, treatment with ultrapure water, and not with plane cells, was used as control. After 24 hours, 10 and 25 μg/mL of AgNPs showed significant increase in tail length, while 50 and 75 μg/mL of AgNPs showed significant increase in all three parameters observed. Treatment with AgNO3 showed significant influence only on the tail length when compared with control. After 48 hours, 5 and 10 μg/mL of AgNPs showed significant increase in tail length, while 25, 50 and 75 μg/mL of AgNPs as well as treatment with AgNO3 showed significant increase in all three parameters when compared with control. Overall, the study showed that AgNP have genotoxic efect on Pk15 cells.

genotoxicity; nanosilver; in vitro; PK15 cells

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