Stable expression of chimeric heavy chain antibodies in CHO cells (CROSBI ID 47693)
Prilog u knjizi | izvorni znanstveni rad
Podaci o odgovornosti
Agrawal, Vishal ; Slivac, Igor ; Perret, Sylvie ; Bisson, Louis ; St-Laurent, Gilles ; Murad, Yanal ; Zhang, Jianbing ; Durocher, Yves
engleski
Stable expression of chimeric heavy chain antibodies in CHO cells
Camelid single domain antibodies fused to noncamelid Fc regions, also called chimeric heavy chain antibodies (cHCAb), offer great potential as therapeutic and diagnostic candidates due to their relatively small size (80 kDa) and intact Fc. In this chapter, we describe two approaches, limiting dilution and minipools, for generating nonamplified Chinese hamster ovary cell lines stably expressing cHCAb in suspension and serum-free cultures using a stringent antibiotic selection. Neither of the protocols necessitates the acquisition or implementation of expensive automated infrastructures and thus could be applied in any lab with minimal cell culture setup. The given protocol allows the isolation of stable clones capable of generating up to 100 mg/L of antibody in batch mode performed in shaker flasks.
Fc fusions, PEImax, Antibody production, Cell line development, Antibiotic selection
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Podaci o prilogu
287-303.
objavljeno
Podaci o knjizi
Single Domain Antibodies : Methods and Protocols
Walker, J.M.
Totowa (NJ): Humana Press
2012.
978-1-61779-968-6