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Stable expression of chimeric heavy chain antibodies in CHO cells (CROSBI ID 47693)

Prilog u knjizi | izvorni znanstveni rad

Agrawal, Vishal ; Slivac, Igor ; Perret, Sylvie ; Bisson, Louis ; St-Laurent, Gilles ; Murad, Yanal ; Zhang, Jianbing ; Durocher, Yves Stable expression of chimeric heavy chain antibodies in CHO cells // Single Domain Antibodies : Methods and Protocols / Walker, J.M. (ur.). Totowa (NJ): Humana Press, 2012. str. 287-303

Podaci o odgovornosti

Agrawal, Vishal ; Slivac, Igor ; Perret, Sylvie ; Bisson, Louis ; St-Laurent, Gilles ; Murad, Yanal ; Zhang, Jianbing ; Durocher, Yves

engleski

Stable expression of chimeric heavy chain antibodies in CHO cells

Camelid single domain antibodies fused to noncamelid Fc regions, also called chimeric heavy chain antibodies (cHCAb), offer great potential as therapeutic and diagnostic candidates due to their relatively small size (80 kDa) and intact Fc. In this chapter, we describe two approaches, limiting dilution and minipools, for generating nonamplified Chinese hamster ovary cell lines stably expressing cHCAb in suspension and serum-free cultures using a stringent antibiotic selection. Neither of the protocols necessitates the acquisition or implementation of expensive automated infrastructures and thus could be applied in any lab with minimal cell culture setup. The given protocol allows the isolation of stable clones capable of generating up to 100 mg/L of antibody in batch mode performed in shaker flasks.

Fc fusions, PEImax, Antibody production, Cell line development, Antibiotic selection

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Podaci o prilogu

287-303.

objavljeno

Podaci o knjizi

Single Domain Antibodies : Methods and Protocols

Walker, J.M.

Totowa (NJ): Humana Press

2012.

978-1-61779-968-6

Povezanost rada

Biotehnologija