engleski

Antiproliferative effects of cytokines on squamous cell carcinoma

A panel of 12 squamous cell carcinoma of the head and neck (SCCHN) cell lines has been used to determine sensitivity of tumor cells to cytokines, tumor necrosis factor alpha (TNF- ), interferon gamma (IFN- ), and interferon alfa (IFN- ) in vitro. Antiproliferative activity of these cytokines on squamous cell carcinoma of the head and neck monolayers was measured in a colorimetric MTT [3-(4, 5-dimethylthiazol-2yl)-2, 5-diphenyl tetrazolium bromide]-based assay. All 12 cell lines tested were sensitive to IFN- , with the 50% inhibitory dose (ID50) ranging from 0.07 ± 0.001 to 104 ± 4.6 U/mL. The TNF- showed antiproliferative activity on three cell lines at relatively high doses (ID50 from 55 ± 4.1 to 847.10 ± 10 U/mL), and IFN- was growth inhibitory in only one line (ID50 = 1211 ± 46.2 U/mL). The combination of IFN- and TNF- had a synergistic antiproliferative effect on eight cell lines and an additive effect on two cell lines. In two cell lines, the effect of the combination was equal to that of IFN- alone. A combination of IFN- and TNF- resulted in cell growth inhibition in six of the seven lines tested, and this effect was synergistic. These in vitro studies indicate that combinations of IFN- and TNF- or IFN- and TNF- may be more growth inhibitory against squamous cell carcinoma of the head and neck and at lower doses than each of these cytokines used singly.

ntiproliferative effect; cytokines; squamous cell carcinoma

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