Diatom Polysaccharides: Extracellular Production, Isolation and Molecular Characterization (CROSBI ID 47646)
Prilog u knjizi | izvorni znanstveni rad
Podaci o odgovornosti
Urbani, Ranieri ; Paola, Sist ; Pletikapić, Galja ; Mišić Radić, Tea ; Svetličić, Vesna ; Žutić, Vera
engleski
Diatom Polysaccharides: Extracellular Production, Isolation and Molecular Characterization
This chapter aims to give an overview on the isolation procedures and techniques for macromolecular characterization of extracellular polysaccharides from laboratory cultures of selected diatom species and from native marine aggregates. The emphasis is given to the macromolecular behavior of isolated polysaccharide in solution and their propensity to aggregate and to form highly structured systems like gels. This was made possible by developing protocols and procedures for isolation/separation and application of physico-chemical techniques like laser light scattering and viscosimetry to marine and culture samples. A separate section is devoted to atomic force microscopy, the most versatile nanotechnology platform widely used in polymer biophysics and diatom nanotechnology. The first force spectra experiments conducted on isolated polysaccharide fibrils and on marine gel polysaccharide network are presented together with recently published works on high resolution imaging. Diatom exopolysaccharide force spectroscopy and interactions with nanoparticles are identified as future prospective contributing to the growing field of nanoecology and for rational design of polysaccharide gels with desired properties.
polysaccharides ; extracellular polysaccharide ; viscometry ; DLS ; atomic force microscopy ; marine gel network ; Northern Adriatic macroaggregates
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Podaci o prilogu
345-367.
objavljeno
10.5772/51251
Podaci o knjizi
The Complex World of Polysaccharides
Desiree Nedra, Karunaratne,
Lahti: IntechOpen
2012.
098-0982934-2744