engleski

Frequencies of hemolysis and lipemia in whole blood samples in pediatric population

Introduction: The aim of this study was to assess prevalence of interference from hemolysis and lipemia in whole blood samples in pediatric population. Materials and methods: 3903 consecutive samples collected during one month period were visually monitored for turbidity and hemolysis and patients age were noted. The statistical significance of differences between relative frequencies of interferences was calculated with χ2 test. Results: 7% of all samples were identified to have hemolysis. Hemolysis in samples drawn from vein occurred more frequently than hemolysis in capillary samples (P < 0.001). 18% of samples were identified to have lipemia. Both lipemia and hemolysis were observed in < 1% of samples. Interferences were more frequent in population up to 2 years old (P < 0.001). Conclusion: Due to presence of interferences in significant number of samples, particularly in population up to 2 years old, a considerable number of whole blood samples may be unreliable. Continuous education and training of healthcare personnel in phlebotomy and patient preparation prior to sample collection is needed.

whole blood sample; hemolysis; lipemia; interference; child

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