engleski

Colonic lesions after cysteamine application in rats. The influence of pentadecapeptide BPC-157

Since the introductory description by Hans Selye and Sandor Szabo in Nature, 1973, cysteamine was in use as the most specific duodenal ulcerogen, applied accordint do different protocols, being one of the most established models in gastrointestinal research. The cysteamin ulcer was regularly considered as a specific result of local factors and it was pertinently used for reliable duodenal ulcer induction, and salutary agents screening. A particular damaging effect not related do gastric acid secretion, and a direct necrotising, "cytotoxic" effect was recently proposed for cysteamin in gastrectomized rats. Thus, it was likely that cysteamine, besides duodenal (and stomach) lesion would induce the lesions in other parts of gastrointestinal tract, when appropriately applied. In the experiment, four groups of rats were used, i.e. two control (C-saline (n=15) ; C1-acified saline with pH=3, 8, which is identical to the acidity of cysteamin (n=30) and two treaated groups (cysteamine 400 mg/kg b.w. intrarectally (n=30) ; cysteamine+BPC 157 (10ug/kg b.w. intragastrically) (n=30). Since the cysteamine would induce large lesion, the colon was removed and a lesion areas (mm2) were assessed by naive observers and morphomoetrical analysis system (using a PC based program SFORM, VAMS, Zagreb, Croatia). Representative tissue sections were processed for further histological analysis in which the intensity of oedema, necrosis, colonic gangliolar destruction, perivascular infiltration, haemorrhage, granulocytic and mononuclear cell infiltration were scored using four grad scoring system (1-no change ; 2-mild ; 3-moderate ; 4-severe). In this study the ulcerogenic effect of the intrarectal administration of the cysteamine on colon mucosa was firstly shown in rats. The salutary effect of a novel stomach pentadecapeptide BPC 157 was noticed against cysteamine colon lesions (assessed after, 30 min, 1 hour, 3 hours, 24, hours, 48 hours and 72 hours following cysteamine in the both treated grous of animals). Acified saline had minor macroscopical and pathohistological changes noticed only in the group sacrificed after 30 min following cysteamine.

cysteamin; colon; rat; animal model

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